塞尼卡病毒A VP1基因遗传进化分析

Phylogenetic Analysis of VP1 Gene of Senecavirus A

[目的]研究塞尼卡病毒A VP1基因的遗传变异情况。[方法]通过RT-PCR方法从广东省2个猪场的病料中扩增出塞尼卡病毒A阳性样品,并对其VP1基因进行了基因组扩增和序列分析。[结果]2个塞尼卡病毒A VP1基因与Gen Bank公布的巴西、美国、中国等毒株的同源性为92%99%,与2002年美国分离株SVV-001的同源性分别为92.8%和92.7%,与我国分离株CH-02-2015、CH-03-2015、CH-04-2015的同源性最高达99.1%。通过序列分析发现,毒株VP1基因组存在散在突变点,表明毒株可能存在一定程度的变异。[结论]研究结果可为我国塞尼卡病毒A的深入研究和猪水疱样症状病的鉴别诊断提供参考。

[Objective]To study the genetic variation of senecavirus A VP1 gene.[Method]The positive samples of SVV in swine with vesicular symptom from two farms of Guangdong were amplified by RT-PCR method.The sequencing and phylogenetic analysis was made on Senecavirus A VP1.[Result]The homology between VP1 gene of these two strains and Brazil,USA and Chinese historical isolates on Gen Bank was 92%-99%.The homology between VP1 of these two strains and USA isolate SVV-001 in 2002 were 92.8% and 92.7% respectively.The highest homology of SVA VP1 gene with Chinese isolates CH-02-2015,CH-03-2015 and CH-04-2015 reached 99.1%.Sequencing analysis results showed that the VP1 gene existed scatter point,which suggested that the virus had evolved.[Conclusion]The research results can provide references for further study and differential diagnosis of SVA.