大豆异黄酮对雄性大鼠脾脏IL-2、IL-4、TNF-α、INF-γ蛋白表达的影响

Effects of soy isoflavones on expression of IL-2,IL-4,TNF-α and INF-γ in male rats' spleen

试验旨在探讨不同剂量大豆异黄酮对雄性大鼠脾脏免疫功能的影响及其机理。将40只6周龄雄性SD大鼠随机分为4组,每组10只。对照组和低、中、高剂量组分别灌胃添加不同水平的大豆异黄酮(0、50、250、500 mg·kg-1),大鼠每周称量体质量,连续干预4周后处死大鼠并记录脾脏质量;利用免疫组织化学链霉亲和素-生物素复合物(SABC)染色法研究大鼠脾脏中白介素-2(IL-2)、白介素-4(IL-4)、肿瘤坏死因子α(TNF-α)及干扰素γ(INF-γ)蛋白的表达水平。结果显示,与对照组相比,各组间脾脏指数差异不显著;中、高剂量组脾小体体积显著减小,红髓区淋巴细胞数量显著减少;IL-2在低剂量组表达显著降低,但在中、高剂量组表达增加且在高剂量组达到显著水平;IL-4、TNF-α和INF-γ在大豆异黄酮干预后各剂量组表达水平均显著降低。综上,高剂量的大豆异黄酮能够提高IL-2蛋白的表达水平,各剂量组总体抑制了IL-4、TNF-α和INF-γ蛋白的表达水平,这表明大豆异黄酮对雄性健康大鼠存在潜在的免疫抑制,且呈现一定的剂量差异。

In the present study,effects of different dose of soy isoflavones（ SIF） on splenic immune function in male rats were studied and mechanism was explained. Forty rats aged 6 weeks were randomly divided four groups fed with SIF 0（ control group）,50（ low dose group）,250（ medium dose group）,500（ high dose group） mg·kg-1 body weight. Body weight were recorded every week,and at the end of experiment the rats＇ spleens were separated and weighed. The expression levels of IL-2,IL-4,TNF-α and INF-γ in rats spleen were detected by immunohistochemistry SABC method. The results showed that compared with control group,there were no significantly differences in spleen index among the groups. Splenic corpuscles in middle and high group were smaller than those in other groups with the lymphocytes number significantly decreased in red pulp. The expression of IL-2 in low dose group was signifi-cantly decreased,but rapidly increased in middle and high dose group with a dose-dependent. However,the expression levels of IL-4,TNF-α and INF-γ were all significantly reduced after treated by isoflavones. All above,even though high dose isoflavones can increase IL-2 level,the expressions of IL-4,TNF-α and INF-γ were inhibited with the dose of isoflavones increasing,which indicated SIF had potential immunosuppression to male rats and appeared dosage difference.