四川部分地区禽源空肠弯曲菌ERIC-PCR分型及毒力相关基因分析

ERIC-PCR typing and virulence-related genes detection of Campylobacter jejuni isolated from poultry in some areas of Sichuan Province

为了解四川地区禽源空肠弯曲菌(Campylobacter jejuni)的遗传多样性和毒力相关基因的携带情况,对从四川成都、雅安及眉山市采集的鸡源、鸭源和鹌鹑源的盲肠及肛拭子样品进行C.jejuni的分离鉴定、ERICPCR分型和17种毒力相关基因的检测分析。结果显示,总体分离率为10.23%(48/469),其中鸡源分离率显著高于鹌鹑源(P<0.05)。ERIC-PCR聚类分析显示48株C.jejuni可分为6簇、16类共31种基因型,不同场分离株多在某一类或某一簇内聚集分布,而部分菌株在不同地区、不同场之间呈交叉分布。毒力基因检测结果显示,15种毒力相关基因的携带率在50%以上,其中鹌鹑源分离株的毒力基因携带率高于其他动物的携带率。由此表明四川地区禽源C.jejuni毒力基因的携带率高并且具有较高的遗传多样性。

In order to investigate the genetic diversity and virulence related genes of Campylobacter jejuni in some areas of Sichuan,the samples of the cecum and anal swab collected from the chickens,ducks and quails from Chengdu,Ya＇an and Meishan were isolated and identified for C. jejuni,and the ERIC-PCR typing and seventeen virulencerelated genes were detected and analyzed. The results of isolation showed a 10. 23% separation rate（ 48/469）,and the isolation rate from chicken was higher than that from quail（ P 〈0. 05）. ERIC-PCR cluster analysis showed that forty-eight strains of C. jejuni could be divided into six clusters,and there were sixteen species and thirty-one genotypes. The isolates from each farm were clustered and distributed in some species or cluster,while some isolates showed cross distribution in different regions and different farms. The carrying rate of fifteen virulence related geneswas above 50%,and the carrying rate of virulence related genes of quail was higher than other animals. The results suggested that the C. jejuni isolates from poultry of Sichuan area possessed high carrying rate and high genetic diversity.