水杨酸和脯氨酸对盐胁迫下生菜相关基因表达的影响

Effect of salicylic acid and proline on gene expression profiles in response to salt stress in lettuce

为了探明水杨酸(SA)和脯氨酸(Pro)在缓解植物盐胁迫中的作用,以生菜品种‘美国大叶速生’为材料,采用营养液水培方式,分别使用盐胁迫水培液以及盐胁迫水培液中分别添加SA和Pro 2种外源物质处理生菜幼苗,并且测定相关形态指标、生理指标以及处理后与盐胁迫逆境相关基因的表达量。研究表明,与对照相比,在盐胁迫下加入0.2 mmol·L^(-1)SA和0.2 mmol·L^(-1)Pro处理24 h时,生菜的形态指标缓解效果明显。半定量PCR与荧光定量PCR研究结果基本一致,同时相关抗逆基因LSNCED1、LSNCED2、LSP5CS的表达量在盐胁迫下都有不同程度的上升。结果表明,添加0.2 mmol·L^(-1)SA和0.2 mmol·L^(-1)Pro处理24 h能够缓解盐胁迫对生菜造成的伤害。

In order to investigate the role of salicylic acid（ SA） and proline（ Pro） in alleviating salt stress in plants,a lettuce cultivar ‘Meiguodayesusheng＇ was used in this study. The lettuce seedlings were grown in hydroponic system under salt stress,and SA and Pro were added to the nutrition solution,respectively. In determination of the morphological and physiological indexes related to salt stress,the results showed that the growth of lettuce seedlings under salt stress was significantly alleviated when treated with 0. 2 mmol·L^（-1） SA and 0. 2 mmol·L^（-1） Pro for 24 hours.The expression levels of the genes related to salt stress were analyzed by RT-PCR and q RT-PCR,and the expression of LSNCED1,LSNCED2 and LSP5 CS were remarkably up-regulated under salt stress. These results indicated that 0. 2 mmol·L^（-1） SA and 0. 2 mmol·L^（-1） Pro could reduce the injury to lettuce caused by salt stress to some extent.