皂荚种质资源SRAP遗传多样性分析及指纹图谱的构建

Genetic diversity and fingerprints of Gleditsia sinensis germplasm based on SRAP

利用SRAP标记技术对18份皂荚种质材料进行了遗传多样性分析。结果表明,从64对SRAP引物中筛选了17对引物进行PCR扩增,共扩增出222个条带,其中多态性条带213个,多态性比率为95.95%。各引物多态性信息含量(PIC)、观测等位基因数(Na)、有效等位基因数(Ne)、Nei's基因多样性指数(H)和Shannon's信息指数(I)的平均值分别为0.861 1、1.962 3、1.415 3、0.257 2和0.406 6,18份皂荚种质资源间遗传相似系数(GS)为0.522 5~0.955 0。UPGMA聚类分析表明,在遗传相似系数为0.66处可将18份皂荚种质资源分为3组,其中,野皂荚单独为1组,山皂荚和皂荚-T聚为1组,其他皂荚材料聚为1组。利用4对引物扩增的9个多态性位点构建了18份皂荚种质资源的DNA指纹图谱,可以将其区分并精准鉴定。该研究结果将为皂荚种质的鉴定、保存和新品种选育提供一定的理论依据。

Genetic diversity of 18 Gleditsia sinensis germplasms were analyzed by SRAP markers. The results showed that 17 primers pairs were screened from 64 primer pairs and 222 bands were obtained,including 213 polymorphic bands,with a polymorphism rate of 95. 95%. The average polymorphism information content（ PIC）,observed number of alleles（ Na）,effective number of alleles（ Ne）,Nei＇s gene diversity（ H） and Shannon＇s information index（ I） were 0. 861 1,1. 962 3,1. 415 3,0. 257 2 and 0. 406 6,and the genetic similarity coefficients（ GS） among the tested samples ranged from 0. 522 5 to 0. 955 0. UPGMA analysis showed that 18 Gleditsia sinensis could clustered into 3 groups with the GS of 0. 66. Gleditsia heterophylla formed the first group,Gleditsia melanacantha and Zaojia-T were classified into the second group,and the others were classified into the third group. A total of 18 Gleditsia sinensis germplasm DNA fingerprints were constructed from 9 polymorphic loci amplified by 4 primer pairs,and these materials could be distinguished and identified accurately. All these results would provide the important theoretical basis for the identification,conservation and breeding of new cultivar for Gleditsia sinensis germplasms.