同时检测食物不耐受特异性IgG抗体和过敏原IgE抗体在儿童支气管哮喘中的价值探讨

The Value of Simultaneous Detection of Food Intolerance Specific IgG Antibodies and Allergen IgE Antibodies in Children with Bronchial Asthma

目的该研究通过对支气管哮喘儿童同时检测食物不耐受特异性IgG抗体和过敏原IgE抗体结果及内在联系进行分析,以期对支气管哮喘发病机理、诊断、治疗及预防儿童支气管哮喘提供有效的指导意见。方法方便选取2015年9月—2017年3月该院儿科确诊的支气管哮喘患儿132例,应用酶联免疫吸附法半定量检测食物不耐受特异性IgG抗体,采用荧光酶联免疫吸附试验(CAP)检测过敏原IgE抗体。结果支气管哮喘患儿食物不耐受特异性IgG抗体阳性率为95.5%(126/132),过敏原IgE抗体阳性率占65.2%(86/132),这两项试验的结果是一致部分的约66.7%(88/132),不一致的约占33.3%(44/132),全阴性是3.0%(4/132);食物不耐受特异性IgG抗体阳性126例中,过敏原IgE抗体同时阳性84例,占66.7%(84/126);过敏原IgE抗体阳性86例中,食物不耐受特异性IgG抗体同时阳性84例,占97.7%(84/86);过敏原IgE抗体阴性46例中,有42例食物不耐受特异性IgG抗体阳性,阳性率占31.8%(42/132),差异有统计学意义(P<0.05)。结论食物不耐受与过敏症在哮喘儿童中共患性高,在哮喘病因诊断中,食物不耐受特异性IgG抗体可作为过敏原IgE抗体检测的强有力补充。

Objective This paper tries to analyze the results and relationship of the detection of food intolerance specific IgG antibody and allergen IgE antibody in children with bronchial asthma, to provide effective guidance for the pathogenesis, diagnosis, treatment and prevention of bronchial asthma in children with bronchial asthma. Methods Convenient selection 132 cases of children with bronchial asthma treated from September 2015 to March 2017 in this hospital were selected.Semiquantitative food intolerance specific IgG antibodies was determinated by enzyme-linked immunosorbent assay, the specific IgE antibody was detected by fluorescence enzyme-linked immunosorbent assay（CAP） in vitro. Results The positive rate of food intolerance and specific IgG antibody in children with bronchial asthma was 95.5%（126/132）, the positive rate of specific IgE antibody was 65.2%（86/132）, the consistent rate of the results in the two tests were about 66.7%（88/132）, inconsistencies accounted for about 33.3%（44/132）, the total negative was 3.0%（4/132）; among the 126 cases of food intolerance specific IgG antibodies, 84 cases were positive allergen specific IgE antibodies, accounting for 66.7%（84/126）;among the 86 cases of allergen specific IgE antibodies, 84 cases of food intolerance specific IgG antibodies were positive,accounting for 97.7%（84/86）. 46 cases of negative allergen specific IgE antibodies, 42 cases of food intolerance specific IgG antibody were positive, and the positive rate was 31.8%（42/132）, the difference was statistically significant（P0.05）. Conclusion Food intolerance and allergies are highly prevalent in asthmatic children, in the diagnosis of asthma etiology, food intolerance specific IgG antibodies may serve as a strong complement to allergen IgE antibody testing.