花菁染料负载的聚合物纳米材料用于次氯酸可视化检测的研究

Cyanine Dye-loading Polymer Nanoparticle for Colorimetric Detection of Hypochlorous Acid

次氯酸(HClO)作为强氧化剂被广泛应用于日常生活中,研究对HClO浓度的便捷监测方法具有重要意义。针对HClO的可视化探针存在水溶性较差和易被光漂白的问题,本工作利用次氯酸根(ClO-)可破坏花菁染料的共轭结构这一特性,设计合成了一种花菁染料Cy7负载的聚苯乙烯纳米球(PS-Cy7),并研究了其与次氯酸的响应行为。实验结果表明,PS-Cy7具有良好的单分散性,粒径约为78nm;相比于花菁染料Cy7,PS-Cy7具有更好的水溶性和光稳定性,并且对次氯酸具有专一性的响应,其最低检测限为0.22μmol/L,实现了对HClO的可视化检测,有望用于日常生活中的HClO监测。

As a strong oxidant,HClO was widely applied in people everyday life,so it is great important to monitor the concentration of HClO.Herein,we developed a new strategy to resolve the deficiency of photobleaching and poor water-solubility for the current HClO probes.On the basis of the fact that hypochlorite can destroy the conjugated structure of the organic dye,a polystyrene nanoparticle loaded with the Cy7 dye （PS-Cy7） had been designed and synthesized.Moreover,PS-Cy7 with the size of 78 nm was carried out as colorimetric nanoprobe for detection of hypochlorite anion （ClO^-）.The nanoprobe exhibits good watersolubility and high photostability.The detection limit of PS-Cy7 for hypochlorite in water was 0.22 μmol/L.Furthermore,PS-Cy7 can specifically respond to hypochlorite in presence of other reactive oxygen species.The experimental result demonstrated that PS-Cy7 was a colorimetric nanoprobe with high sensitivity and selectivity of hypochlorite.