原发性肝癌患者超声剪切波速度与癌细胞生长、病灶内血管新生的相关性研究

Correlation of ultrasonic shear wave velocity with cancer cell growth and intralesional angiogenesis in patients with primary liver

目的:研究原发性肝癌患者超声剪切波速度(SWV)与癌细胞生长、病灶内血管新生的相关性。方法:选择在本院诊断为原发性肝癌的患者,进行实时剪切波弹性成像检查并测定超声剪切波速度;采集肝癌病灶及癌旁病灶并检测抑癌基因、增殖基因、血管新生基因的表达量。结果:肝癌病灶内PICK1、Merlin、PTEN、FN的蛋白表达量均显著低于癌旁病灶,STC2、N-cadherin、Bcl-2、CyclinD1、E2F、Gli2、VEGFA、MMP9、Ang-2的蛋白表达量均显著高于癌旁病灶;肝癌病灶的SWV与PICK1、Merlin、PTEN、FN的蛋白表达量呈负相关,与STC2、N-cadherin、Bcl-2、CyclinD1、E2F、Gli2、VEGFA、MMP9、Ang-2的蛋白表达量呈正相关。结论:原发性肝癌患者SWV的增高与癌细胞生长活力及血管新生增加密切相关。

Objective：To study the correlation of ultrasonic shear wave velocity（SWV）with cancer cell growth and intralesional angiogenesis in patients with primary liver cancer.Methods：Patients who were diagnosed with primary liver cancer in Xuzhou Central Hospital were selected,and real-time shear wave elastography was conducted to determine the ultrasonic shear wave velocity;liver cancer lesions and adjacent lesions were collected to determine the expression of tumor suppressor genes,proliferation genes and angiogenesis genes.Results：PICK1,Merlin,PTEN and FN protein expression in liver cancer lesions were significantly lower than those in adjacent lesions,while STC2,N-cadherin,Bcl-2,CyclinD1,E2 F,Gli2,VEGFA,MMP9 and Ang-2protein expression were significantly higher than those in adjacent lesions;liver cancer SWV was negatively correlated with PICK1,Merlin,PTEN and FN protein expression,and positively correlated with STC2,N-cadherin,Bcl-2,CyclinD1,E2 F,Gli2,VEGFA,MMP9 and Ang-2protein expression.Conclusion：The increase of SWV in patients with primary liver cancer is closely related to the cancer cell growth activity and the increase of angiogenesis.