套细胞淋巴瘤中PI3K/Akt信号通路与细胞凋亡、侵袭的相关性

Correlation of PI3K/Akt signaling pathway with cell apoptosis and invasion in mantle cell lymphoma

目的:研究套细胞淋巴瘤中PI3K/Akt信号通路与细胞凋亡、侵袭的相关性。方法:选择在本院诊断为套细胞性淋巴瘤的38例患者作为研究的MCL组,同期在第四军医大学附属西京医院诊断为淋巴结反应性增生的55例患者作为研究的对照组,采集淋巴结组织,检测p-PI3K、p-AKT的蛋白表达量以及凋亡基因、侵袭基因的mRNA表达量。结果:MCL组患者淋巴结中p-PI3K、p-AKT的蛋白表达量以及SOX11、cyclinD1、TNFAIP3、XIAP、PCNA、MMP2、MMP7、MMP9、VEGF的mRNA表达量均显著高于对照组,TNFAIP3的mRNA表达量均显著低于对照组;p-PI3K高表达的MCL淋巴结中SOX11、cyclinD1、XIAP、PCNA、MMP2、MMP7、MMP9、VEGF的mRNA表达量均显著高于p-PI3K低表达的MCL淋巴结,TNFAIP3的mRNA表达量均显著低于p-PI3K低表达的MCL淋巴结。结论:套细胞淋巴瘤中PI3K/Akt信号通路的激活与肿瘤细胞凋亡障碍、侵袭增强密切相关。

Objective：To study the correlation of PI3K/Akt signaling pathway with cell apoptosis and invasion in mantle cell lymphoma.Methods：A total of 38 patients who were diagnosed with mantle cell lymphoma in Xijing Hospital Affiliated to the Fourth Military Medical University between June 2014 and March 2017 were selected as the MCL group of the research,and 55 patients who were diagnosed with reactive lymphoid hyperplasia in this hospital during the same period were selected as the control group.Lymph node tissue was collected to detect the protein expression of p-PI3 Kand p-AKT as well as the mRNA expression of apoptosis genes and invasion genes.Results：p-PI3 Kand p-AKT protein expression as well as SOX11,cyclinD1,TNFAIP3,XIAP,PCNA,MMP2,MMP7,MMP9 and VEGF mRNA expression in lymph node of MCL group were significantly higher than those in control group while TNFAIP3 mRNA expression was significantly lower than that in control group;SOX11,cyclinD1,XIAP,PCNA,MMP2,MMP7,MMP9 and VEGF mRNA expression in MCL lymph node with high pPI3 Kexpression were significantly higher than those in MCL lymph node with low p-PI3 Kexpression while TNFAIP3 mRNA expression was significantly lower than that in MCL lymph node with low p-PI3 Kexpression.Conclusions：The activation of PI3K/Akt signaling pathway in mantle cell lymphoma is closely related to the tumor cell apoptosis disorder and invasion enhancement.