浸润性乳腺癌MRI表观扩散系数与肿瘤组织生长、新生血管生成的相关性

Correlation of MRI apparent diffusion coefficient of invasive breast cancer with tumor tissue growth and angiogenesis

目的:研究浸润性乳腺癌MRI表观扩散系数(ADC值)与肿瘤组织生长、新生血管生成的相关性。方法:选择在本院就诊的乳腺肿块患者作为研究对象,根据穿刺活检结果分为浸润性导管癌的A组、导管内癌的B组以及良性病变的C组,进行磁共振扩散加权成像并测定ADC值,取穿刺活检组织并检测增殖基因、血管新生基因的表达量。结果:A组和B组患者病灶中USP39、CyclinD1、VEGF、bFGF、Angplt-2、Angplt-3、Angplt-4的蛋白表达量均显著高于C组,ADC值及ALEX1、Bax的蛋白表达量均显著低于C组;A组患者病灶中USP39、CyclinD1、VEGF、bFGF、Angplt-2、Angplt-3、Angplt-4的蛋白表达量均显著高于B组,ADC值及ALEX1、Bax的蛋白表达量均显著低于B组;高ADC值浸润性乳腺癌组织中USP39、CyclinD1、VEGF、bFGF、Angplt-2、Angplt-3、Angplt-4的蛋白表达量均显著低于低ADC值浸润性乳腺癌组织,ALEX1、Bax的蛋白表达量均显著高于低ADC值浸润性乳腺癌组织。结论:浸润性乳腺癌ADC值的降低与癌细胞增殖、新生血管生成密切相关。

Objective：To study the correlation of MRI apparent diffusion coefficient（ADC value）of invasive breast cancer with tumor tissue growth and angiogenesis.Methods：Patients with breast mass who were treated in Wuhan No.6Hospital were selected as the research subjects,patients were divided into group A with invasive ductal carcinoma,group B with intraductal carcinoma and group C with benign lesion according to the biopsy results.Magnetic resonance diffusion-weighted imaging was conducted to determine ADC values,and biopsy tissue was taken to determine the expression of proliferation genes and angiogenesis genes.Results：USP39,CyclinD1,VEGF,bFGF,Angplt-2,Angplt-3and Angplt-4protein expression levels in lesions of group A and group B were significantly higher than those of group C,while ADC value as well as ALEX1 and Bax protein expression levels were significantly lower than those of group C;USP39,CyclinD1,VEGF,bFGF,Angplt-2,Angplt-3and Angplt-4protein expression levels in lesions of group A were significantly higher than those of group B,while ADC value as well as ALEX1 and Bax protein expression levels were significantly lower than those of group B;USP39,CyclinD1,VEGF,bFGF,Angplt-2,Angplt-3and Angplt-4protein expression levels in invasive breast cancer tissue with high ADC value were significantly lower than those in invasive breast cancer tissue with low ADC value,while ALEX1 and Bax protein expression levels were significantly higher than those in invasive breast cancer tissue with low ADC value.Conclusion：The decrease of ADC value of invasive breast cancer is closely related to cancer cell proliferation and angiogenesis.