高温高湿下辣椒抗病分子机制及其遗传改良技术与应用

Molecular mechanism of disease resistance for pepper and its genetic improvement measures and application under hot and humid environment

高温高湿环境下病害严重发生是导致我国南方辣椒露地栽培和设施栽培减产的重要障碍因素。该文对辣椒应答高温高湿胁迫和病原菌侵染的分子机制进行研究,发现了以CaWRKY40为中心的转录调控网络在辣椒耐高温、抗病及高温高湿下抗病表现中起重要的调节作用。在此基础上建立了病圃与高温高湿处理相结合的生态压力选择法与CaWRKY40转录表达量相结合的资源筛选体系,对通过有性杂交、诱变等创制的中间材料实施在高温高湿环境下的抗病资源筛选与新品种选育,选育出的辣椒新品种均表现出在高温高湿环境下较高的抗病水平,适合在我国南方地区进行露地栽培或设施栽培。

Severe disease damage under hot and humid environment is the important factor which reduces the yield of pepper cultivated in open field and greenhouse in south of our country.In this study,the molecular mechanism for pepper response to high temperature and humidity and pathogen infection was studied,and the functions of CaWRKY40 transcription and regulation in pepper heat-tolerance,disease-resistance,and disease-resistance under high temperature and humidity condition had been summayized.On the above basis,the pepper varieties selection were established by ecological pressure selection,based on diseased nursery and high temperature and humidity treatment,combined with levels of CaWRKY40 transcription and expression.A series of new Minjiao pepper varieties were bred by selection on various pepper germplasm resources obtained by sexual hybridization and mutation,and all of them were highly resistant to diseases under hot and humid environment,which could be applied in south China in open and protected cultivation.