猫爪草对肺结核患者外周血淋巴细胞颗粒裂解肽表达及其T淋巴细胞杀菌能力的影响

The Effect of Radix Ranuncoli Ternati on the Expression of Granule Lytic Peptide and Bactericidal Ability of T Lymphocyte in Peripheral Blood Lymphocytes of Patients with Pulmonary Tuberculosis

目的研究猫爪草对肺结核患者外周血淋巴细胞颗粒裂解肽表达的影响及其对肺结核患者机体细胞毒性T淋巴细胞杀菌能力的影响。方法四甲基偶氮唑蓝(MTT)检测猫爪草对外周血淋巴细胞作用的最适浓度;Real-time PCR方法检测最适剂量的猫爪草对结核病人外周血淋巴细胞颗粒裂解肽mRNA表达水平的影响。结果 MTT结果显示,当猫爪草的作用浓度为100mg·L^(-1)时,有很明显的诱导作用,能诱导结核菌表达,外周血淋巴细胞颗粒裂解肽(GLS)的表达升高,进而感染结核病人体内的结核菌;当药物浓度浓度为200 mg·L^(-1)时能高效诱导结核休眠菌活化,外周血淋巴细胞GLS mRNA的表达持续升高,对结核菌的杀伤能力更强。结论猫爪草可能通过提高颗粒裂解肽的表达,增强机体对致病菌的杀伤作用,从而达到治疗效果。

OBJECTIVE To explore the effect of Radix Ranuncoli Ternati on the expression of granule lytic peptide and bacteri- cidal ability of T lymphocyte in peripheral blood lymphocytes of patients with pulmonary tuberculosis. METHODS Peripheral blood lymphocytes were treated with Ranunculus . MTT assay was used to detect cell proliferation. Real-time PCR was used to detect the mRNA level of GLS in patients. RESULTS Radix Ranuncoli Ternati could enhance expression of Tubercle bacillus. The results of MTI＂ assay indicated that the rate of peripheral blood lymphocytes apoptosis inducted by the Radix Ranuncoli Ternat. The Western blot- ting results showed that Radix Ranuncoli Ternati made the expression levels of GLS in peripheral blood lymphocyte increasing. When the concentration of the drug is 200 mg ~ L- 1, it can effectively induce the activation of tuberculosis resting bacteria. The expression of GLS and the level of mRNA were increased in peripheral blood lymphocytes. CONCLUSION Radix Ranuncoli Ternati may increase the expression of GLS and enhance the body＇s killing effect on pathogenic bacteria to achieve the therapeutic effect.