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鸡IL-6基因外显子4的克隆及多态性分析

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摘要 为了研究鸡白介素-6(Interleukin-6,IL-6)抗病基因外显子4在群体中的多态性,试验采用测序法和群体遗传学方法进行数据分析,提取150日龄吉林高脚芦花鸡、吉林矮脚芦花鸡、吉林黑鸡肝脏DNA,依据Gen Bank中鸡IL-6基因序列扩增外显子4。结果表明:外显子4第76位氨基酸有A>G的突变,共得到AA、AB、BB三种基因型。纯合度(Ho)分别为0.535,0.671,0.556,杂合度(He)分别为0.465,0.329,0.444,有效等位基因数(Ne)分别为1.869,1.490,1.799,多态信息含量(PIC)分别为0.345,0.275,0.346。
出处 《黑龙江畜牧兽医》 CAS 北大核心 2017年第10期115-117,292,共4页 Heilongjiang Animal Science And veterinary Medicine
基金 吉林省畜禽品种培育项目(吉牧联发[2016]2号) 家养动物种质资源平台项目
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