基于质谱的定量蛋白质组学技术发展现状

Advancements in Quantitative Proteomics Technologies Based on Mass Spectrometry

蛋白质组定量分析技术是支撑蛋白质组学研究的关键技术之一,随着蛋白质组定量分析技术的发展,基于质谱的定量蛋白质组学已成为蛋白质组学研究的重要分支。蛋白质组学定量技术可分为非靶向定量和靶向定量两类,靶向定量技术有MRM和PRM模式,非靶向定量技术有非标记定量和体内外标记定量模式,目前使用最多的同位素标记试剂是i TRAQ和TMT。蛋白质组定量技术按数据采集模式还可分DDA和DIA两类。通过对国内外相关文献收集和分析,系统介绍了蛋白质组质谱定量技术的主要特点和发展现状,旨在为生命科学研究者更好地应用定量蛋白质组学技术提供帮助。

Quantitative proteomic analysis is one of the key technologies in proteomics research. With the development of quantitativetechniques,quantitative proteomics based on mass spectrometry has become an important branch of proteomics research. Quantitativeproteomics technology is generally classified into two categories,untargeted quantitative proteomics and targeted quantitative proteomics.Targeted quantitative technology includes MRM and PRM models,and untargeted quantitative technology includes label-free and in vivo orvitro labeling. The most commonly used isotope labeling reagents are i TRAQ and TMT. According to the data collection model,the quantitativetechnology can be grouped into DDA and DIA. Through the collection and analysis of relevant literature,this article systematically introducesthe main characteristics and current situation of quantitative proteomics based on mass spectrometry,aiming at providing help for theresearchers on life science to better apply the quantitative proteomics technologies.