亚硒酸钠与核黄素联合暴露对高脂饮食大鼠血脂及血清肝生化指标的影响

Effects of combined supplementation by sodium selenite and riboflavin on blood lipid and liver biochemical indicators in rats with high-fat diet

目的研究亚硒酸钠与核黄素联合暴露对高脂饮食雄性大鼠血脂及血清肝生化指标的影响。方法将60只健康SPF级雄性SD大鼠按体重随机分为空白对照组(10只)和高脂组(50只),分别给予基础饲料和高脂饲料;喂养4周后,将50只高脂组大鼠按照血脂水平和体重随机分为5组,分别为高脂对照组、1.84μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组、18.40μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组、1.84μg/kg亚硒酸钠+3.50 mg/kg核黄素干预组和18.40μg/kg亚硒酸钠+3.50 mg/kg核黄素干预组,每组10只。采用灌胃方式进行染毒,同时,空白对照组和高脂对照组给予相同体积的生理盐水,染毒容量为10 ml/kg,每天1次,连续60 d。分别于第0(染毒前)、20、40、60天,测定大鼠血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)水平;并于第60天,检测大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转氨酶(AST)的水平。结果与空白对照组比较,染毒期间高脂对照组及各干预组大鼠血清TC、TG、LDL-C的水平均升高,除第60天时1.84μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组大鼠血清TG水平外,差异有统计学意义(P<0.05);而血清HDL-C水平均未见显著变化。与高脂对照组相比,第20、40天时1.84μg/kg亚硒酸钠+0.70mg/kg核黄素干预组大鼠血清TC、TG的水平及1.84μg/kg亚硒酸钠+3.50 mg/kg核黄素干预组大鼠血清TG的水平以及第60天时1.84μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组大鼠血清TC、TG、LDL-C的水平及18.40μg/kg亚硒酸钠+0.70mg/kg核黄素干预组大鼠血清TC的水平均下降,差异有统计学意义(P<0.05);而各干预组大鼠血清HDL的水平均无明显改变。与空白对照组比较,高脂对照组、18.40μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组和1.84μg/kg亚硒酸钠+3.50mg/kg核黄素干预组大鼠血清ALT的水平以及高脂对照组、18.40μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组和18.40μg/kg亚硒酸钠+3.50 mg/kg核黄素干预组大鼠血清AST的水平均升高,差异有统计学意义(P<0.05)。与高脂对照组相比,仅1.84μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组大鼠血清ALT的水平降低,差异有统计学意义(P<0.05);1.84μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组、18.40μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组以及1.84μg/kg亚硒酸钠+3.50 mg/kg核黄素干预组大鼠血清AST的水平降低,差异均有统计学意义(P<0.05)。结论适量亚硒酸钠与核黄素联合暴露可以拮抗高脂饮食所致雄性大鼠血脂及血清肝生化指标的升高。

Objective To understand the effects of combined supplementation by sodium selenite and riboflavin on blood lipid levels and liver biochemical indicators in rats with high-fat diet. Methods A total of 60 male SD rats were divided into highfat group（50 rats in the group） and control group（10 rats in normal diet）. After fed by high-fat diet for four weeks, all rats in high-fat group were divided into five groups randomly by body weights and blood lipid level, including high-fat control group（saline）,1.84 μg/kg body weight sodium selenite ＋0.70 mg/kg body weight riboflavin intervention group,18.40 μg/kg body weight sodium selenite ＋0.70 mg/kg body weight riboflavin intervention group,1.84 μg/kg body weight sodium selenite ＋3.50mg/kg body weight riboflavin intervention group and 18.40 μg/kg body weight sodium selenite ＋3.50 mg/kg body weight riboflavin intervention group. All rats were treated by gavage for 60 days. Control group was given saline. The serum of rats was collected at the 20 th,40th,and 60 th day after treatment for measurements of blood lipid,and the liver biochemical indicators were determined at the 60 th day of treatment. Results Compared with the control group,the levels of serum TC,TG and LDL-C in all of intervention groups and high-fat control group were significantly increased in all of experiment stages except the level of serum TG in 1.84 μg/kg body weight sodium selenite＋0.70 mg/kg body weight riboflavin intervention group at the 60 th day of treatment（P 〈0.05）. The levels of serum TC,TG in 1.84 μg/kg body weight sodium selenite ＋0.70 mg/kg body weight riboflavin intervention group and the level of serum TG in 1.84 μg/kg body weight sodium selenite ＋3.50 mg/kg body weight riboflavin intervention group were significantly lower than high-fat control group at the 20 th and 40 th day of traetment（P〈0.05）;The levels of serum TC,TG and LDL-C in 1.84 μg/kg body weight sodium selenite ＋0.70 mg/kg body weight riboflavin intervention group and the level of serum TC in 18.4 μg/kg body weight sodium selenite ＋0.70 mg/kg body weight riboflavin intervention group were significantly declined at the 60 th day of treatment（P〈0.05）. Compared with the control group and highfat control group,the level of serum HDL-C in all of intervention groups showed no significantly difference in all of experiment stages（P〉0.05）. Compared with the control group,the levels of serum ALT in high-fat control group,18.4 μg/kg body weight sodium selenite＋0.70 mg/kg body weight riboflavin intervention group and 1.84 μg/kg body weight sodium selenite＋3.50 mg/kg body weight riboflavin intervention group were significantly higher（P〈0.05）; The levels of serum AST in high-fat control group,18.4 μg/kg body weight sodium selenite ＋0.70 mg/kg body weight riboflavin intervention group and 18.4 μg/kg body weight sodium selenite＋3.50 mg/kg body weight riboflavin intervention group were significantly increased（P〈0.05）. The level of serum ALT in 1.84 μg/kg body weight sodium selenite ＋0.70 mg/kg body weight riboflavin intervention group was significantly lower than high-fat control group（P 〈0.05）; The levels of serum AST in 1.84 μg/kg body weight sodium selenite ＋0.70 mg/kg body weight riboflavin intervention group,18.4 μg/kg body weight sodium selenite ＋0.70 mg/kg body weight riboflavin intervention group and 1.84 μg/kg body weight sodium selenite ＋3.50 mg/kg body weight riboflavin intervention group were significantly declined（P 〈0.05）. Conclusion The level of AST,ALT and blood lipid in male rats can be reduced by supplementation of suitable amount of sodium selenite and riboflavin.