COPD患者支气管肺泡灌洗液中IL-22的检测及意义

Detection and Significance of IL-22 in Bronchoalveolar Lavage Fluid of Patients with COPD

目的:检测慢性阻塞性肺病(COPD)患者急性肺炎链球菌感染后支气管肺泡灌洗液(BALF)IL-22的含量,并探讨其临床意义。方法:采用酶联免疫吸附试验(ELISA)检测30例急性肺炎链球菌感染的COPD患者(COPD组)和15例急性肺炎链球菌感染性支气管炎(SP)患者(SP组)BALF上清中IL-22的含量,并对10例患者进行了治疗后跟踪检测,另选取15例健康志愿者作为健康对照组(HC组)。结果:COPD组、SP组患者BALF中IL-22的含量均明显高于健康对照组(P<0.001);且SP组患者BALF中IL-22的含量明显高于COPD组(P<0.01)。治疗后,COPD组患者BALF中IL-22的含量明显下降,吸烟患者IL-22的含量明显高于不吸烟患者(P<0.01)。COPD患者IL-22的含量与中性粒细胞在BALF中的比例呈正相关关系(P<0.01)。COPD患者IL-22的含量与FEV1%呈负相关关系(P<0.01)。结论:COPD患者BALF中IL-22的含量增加与COPD的发生与发展密切相关。

Objective： To investigate the level of IL-22 in BALF of patients with COPD infected pneumonia streptoeoeeus and explore its clinical significance.Method ： Enzyme linked immunosorbent assay （ ELISA ） was used to deteet the content of BALF in the supernatant of IL-22 in 30 patients with COPD （ COPD group ） and 15 patients with aeute streptoeoeeus pneumoniae （ SP ） （ SP group ） infection bronchitis, and 10 patients were followed up after treatment, 15 healthy volunteers served as healthy control group （HC group ） .Result： The content of IL-22 in BALF of COPD group and SP group were significantly higher than that in healthy control group（ P〈0.O01 ）; the content of IL-22 in BALF of SP group was significantly higher than that in COPD group （ P〈0.01 ） .After treatment, the content of IL-22 in BALF of COPD group decreased obviously, and the content of IL-22 in smokers was higher than that in non smokers （ P〈0.01 ） .There was a positive correlation between the content of IL-22 and the proportion of neutrophils in BALF of COPD patients （ P〈0.01 ） .The content of IL-22 in patients with COPD was negatively correlated with FEV1% （ P〈0.01 ） .Conclusion： In patients with COPD, the increase of IL-22 in BALF is closely related to the occurrence and development of COPD.