摘要
生长素响应因子(auxin response factor,ARF)基因家族是植物特有的转录因子家族,在调控植物生长发育过程中起重要作用。以公布的甘蓝基因组数据库为基础,采用生物信息学方法鉴定出29个ARF基因,命名为BoARFs,研究分析了其基因结构、染色体分布、蛋白质保守结构域、系统进化树及表达模式,以期为甘蓝ARF基因家族功能的深入研究提供参考依据。结果表明:BoARF家族基因结构相对复杂,含有2~14个外显子;在染色体上呈不均匀分布,除BoARF29基因未定位到染色体上外,其它基因均定位在不同的染色体上;所有BoARF蛋白均具有保守的B3结构域和Auxin_resp结构域,但只有21个BoARF蛋白包含1~2个AUX/IAA结构域;转录组数据表达模式分析表明,ARF基因具有不同的组织表达模式,部分基因表现出组织特异性。
Auxin response factor（ARF）gene family is the specific transcription factor in plant,and plays an important role in regulation of plant organ development.Based on the published genomic database of Brassica oleracea,29 ARFgenes were identified by bioinformatics,named BoARFs.Their genetic structure,chromosome distribution,conservative domain structure of protein,phylogenetic tree and expression pattern of reference were analyzed for the further research of Brassica oleracea ARF gene family function to provide the reference basis.The results showed that the gene structure of BoARFfamily was relatively complex and contained 2-14 exons.Expect the BoARF29,all other genes were located in different chromosomes.All BoARF proteins had conserved B3 domain and Auxin_resp domain,while only 21 BoARF proteins contained 1 or 2 AUX/IAA domains.Transcriptome data analysis showed that ARFgene had different tissue expression patterns,and some genes showed tissue specificity.
出处
《北方园艺》
CAS
北大核心
2017年第19期16-24,共9页
Northern Horticulture
基金
广东省科技计划资助项目(2015A020209117)
关键词
甘蓝
ARF
生物信息学
Brassica oleracea
auxin response factor
bioinformatics
