同源异型盒基因10蛋白在大肠腺癌中的表达及其临床意义

The expression of HOXD10 protein in colorectal cancer and its clinical significance

目的探讨大肠癌和正常大肠黏膜组织中同源异型盒基因10(HOXD10)的表达情况并探讨其临床意义。方法应用SP免疫组化法检测53例经4%中性甲醛固定和石蜡包埋的大肠癌及53例正常大肠黏膜组织标本中HOXD10蛋白的表达情况,并分析其与大肠癌患者临床病理参数间的关系。结果HOXD10蛋白在正常大肠黏膜组织中的阳性染色率(5.7%)明显低于大肠癌组织(64.2%),两者间差异有统计学意义(P<0.05)。在大肠癌组织中,HOXD10蛋白染色阳性率在高分化(53.8%)、T1+T2(38.5%)、Ⅰ+Ⅱ期(54.3%)、无淋巴结转移(55.3%)均低于在低分化(73.0%)、T3+T4(72.5%)、Ⅲ+Ⅳ期(83.3%)、有淋巴结转移(86.7%),差异均有统计学意义(均P<0.05),但HOXD10蛋白的染色阳性率与大肠癌患者的性别、年龄、癌灶原发部位及癌灶大小无相关性(均P>0.05)。结论 HOXD10蛋白的表达与人大肠癌浸润和转移存在密切相关。

Objective To investigate the expressions of homeobox gene 10 （HOXD10） and analyze its clinical significance. Methods Expressions of HOXD10 protein was detected by immunohistochemistry SP method in 53 cases of colorectal carcinoma tissues and corresponding normal tissues which was fixed by 4% formalin and embedded by paraffin.It was analyzed that the relationship between the expression of HOXD10 protein and clinico- pathological features. Results The positive staining rate of HOXDIO protein in normal coloreetal mucosal tissue （5.7%） was significantly lower than that incolorectal carcinoma tissue （64.2%）, the difference was statistically sig- nificant （P 〈 0.05 ）. In coloreetal cancer tissue, the positive rate of HOXD 10 protein in high differentiation （53.8%）, T1 ＋ T2 （38.5%）, Ⅰ ＋ Ⅱ （54.3%） and no lymph node metastasis （55.3%）was lower than that in low differentiation （ 73.0% ） , T3＋T4 （72.5%） , Ⅲ ＋Ⅳ （ 83.3% ） and lymph node metastasis, the difference was statistically significant （P 〈 0.05）. However, it was not statistically significant between the positive rate of HOXD10 protein and the gender, age, primary site and tumor size in colorectal cancer patients （P 〉 0.05）. Conclusion The expression of HOXD10 protein is closely related to the invasion and metastasis of colorectal cancer.