摘要
以固定化脂肪酶TL IM为催化剂,采用甘油解法在无溶剂体系中制备富含甘油二酯的大豆油。利用气质联用检测甘油二酯含量。考察底物摩尔比、反应温度、反应时间和加酶量对甘油解反应影响,利用响应面试验设计优化各主要因子,并经回归分析获得最优的工艺条件为:加酶量2.0 g,水解温度50℃,水解时间16 h,大豆油与甘油的摩尔比为5:1。在最佳工艺条件下验证,得到大豆油中甘油二酯含量为17.04%。所得回归模型拟合情况良好,达到设计要求。
Glycerol diester-enriched soybean oil was prepared by immobilized lipase TL IM as a catalyst in a solvent-free system using glycerolysis. The content of diacylglycerol was detected by GC/MS. The main factors were optimized by response surface design. The optimal conditions were as follows emzyme amount 20.0 g, hydrolysis temperature 50 ℃, hydrolysis time 16 h, soybean oil and glycerol molar ratio of 5:1. Under the optimum conditions, the content of diglyceride in soybean oil was 17.04%. The obtained regression models could fit well and meet the design requirements.
作者
吴琼
刘晓娟
张姗姗
WU Qiong LIU Xiao-juan ZHANG Shan-shan(China Agricultural Science and Technology Northeast Innovation Center, Changchun 130033 Department of Food Science and Engineering, Key Laboratory of Agricultural Products Processing of Jilin Province Ordinary University, Changchun University, Changchun 130022)
出处
《食品科技》
CAS
北大核心
2017年第9期177-181,共5页
Food Science and Technology
基金
吉林省科技发展计划项目(20140520180JH)