CALR基因对细胞生长和凋亡的调控

Regulation of CALR Gene on Cell Growth and Apoptosis

本研究探讨了CALR基因对细胞生长和凋亡的影响,以MDS患者的肿瘤细胞为研究对象,构建CALR-RNAi慢病毒载体,观察其对SKM-1细胞生长和凋亡的影响。结果证实,MDS患者CALR基因表达较对照组明显降低(p<0.05)。成功构建的3种CALR-RNAi慢病毒载体中,CALR-RNAi(3)慢病毒载体敲除效率最高,转染SKM-1后可促进细胞生长、抑制细胞凋亡(p<0.05),同时CALR-RNAi(3)组的G0/G1期细胞减少,S期细胞增多。CALR基因在MDS的发病及发展中扮演着抑癌基因的角色,其突变及缺失是患者发病的主要原因,关于本基因序列的深入研究对于MDS的临床治疗有重大意义。

We studied the influence of CALR gene on cell growth and apoptosis.We selected tumor cells in MDS patients as the research object,constructed RNAi lentiviral vector targeting CALR gene in SKM-1 to investigate the effect of CLAR-RNAi on proliferation and apoptosis of MDS cells.The results showed that CALR gene expression was significantly decreased in MDS patients when compared with control groups（p〈0.05）.Three kinds of CALR-RNAi-expressing lentiviral vectors were successfully constructed,and the CALR-RNAi（3） lentiviral vector was the most effective vector for interfering the expression of CALR gene.CALR-RNAi（3） could promote cell growth and inhibit apoptosis in SKM-1 cells（p〈0.05）.The percentage of SKM-1 cells in G0/G1 phase was decreased and it was increased in S phase.CALR gene played an important role of tumor suppressor gene in the pathogenesis and developing of MDS,whose mutation and loss was a major cause of illness and had important significance for the treatment of MDS of further research of gene sequence.