钙效应剂对盐胁迫下香蕉叶片结构和生理的影响

Effects of Different Calcium Effectors on Leaf Anatomical Structure and Physiology of Banana under Salt Stress

内为了解香蕉幼苗叶片结构和生理情况,采用在人工模拟盐胁迫环境下,利用EGTA(Ca^(2+)螯合剂)、TFP(CaM拮抗剂)、LaCl_3(Ca^(2+)通道阻碍剂)、外源Ca^(2+)等试剂来阻碍或增强巴西蕉和粉蕉幼苗钙信号转导途径,测定两个不同品种香蕉幼苗叶片的相对含水量、叶绿素含量以及质膜透性,同时通过制作石蜡切片对叶片解剖结构进行了观察。结果表明,盐胁迫下,EGTA、TFP、LaCl_3等钙信号阻碍剂处理使巴西蕉和粉蕉叶片相对含水量、叶绿素含量显著降低,且使其质膜透性显著增加,加剧了叶片组织结构破坏,盐伤害加重,而粉蕉抗性强于巴西蕉,其抗性更敏感,但在盐胁迫下,外源钙能维持细胞外形促使香蕉幼苗叶片正常,使盐胁迫的伤害得到缓解。说明钙信号系统对外源钙缓解盐胁迫对香蕉叶片的伤害有重要的影响。

In order to understand the effects of Calcium effectors on physiological properties and leaves anatomical structure of banana seedlings under salt stress, the Brazil banana （Musa AAA cavendish var. Brazil） and Fenjiao （M. A BB Pisang awak） seedlings were hydroponically cultured. After being blocked or enhanced Ca^2＋ signal transduction treated with Calcium effector EGTA, TFP and LaC13 and exogenous calcium, the effects on banana seedling of two different genotypes under salt stress were studied, including the relative water content （RWC）, chlorophyll content and plasmamembrane permeability of leaves. And through the production of paraffin sections, the anatomical structures of banana seedling leaves were observed as well. The result showed that after treated by Calcium effector EGTA, TFP and LaC13, the relative water content （RWC） and chlorophyll content in leaves of the Brazil banana and Fenjiao seedling under salt stress was significantly decreased, but the plasmamembrane permeability of leaves was significantly increased. The salt stress damage was aggravated, which made leaves anatomical structure of banana suffer more damage. And fenjiao was more sensitive to Calcium effector, of which resistance was stronger than Brazil banana. Exogenous calcium was conducive to maintain normal cellular anatomical structure of banana leaves. And the damages of salt stress to Brazil banana seedling could be mitigated in a certain degree by applying calcium. It suggested that the calcium signaling system had very important effect on exogenous calcium alleviate damage under salt stress of banana leaves.