推拿对腰椎间盘突出症大鼠DRG神经元P2X_3受体影响的实验研究

Experimental Study of Tuina on DRG Neurons P2X3 Receptor of Lumbar Disc Herniation Rats

目的:观察推拿对腰椎间盘突出症大鼠DRG神经元P2X_3受体的影响,探讨推拿对腰椎间盘突出症镇痛效应的外周机制。方法:32只SD大鼠随机分为空白组、模型组、假手术组、推拿组。空白组不做任何处理,模型组和推拿组均将"L"形不锈钢柱插入椎间孔对DRG造成恒定压迫,假手术组除不将"L"形不锈钢柱插入椎间孔外,其余造模步骤同模型组。推拿组在造模后第4天开始推拿手法治疗,其余组均不做治疗。疗程结束后,检测大鼠机械反应阈值(PWT)、缩爪反应潜伏期(PWL),采用Western Blot方法观察DRG神经元P2X_3受体含量的变化。结果:空白组、假手术组大鼠在造模后PWT、PWL均没有显著性差异(P>0.05),模型组大鼠造模后PWT、PWL均显著降低(P<0.001),推拿治疗后与模型组相比较具有显著性差异(P<0.05)。与空白组、假手术组相比,模型组大鼠DRG神经元P2X_3受体含量明显升高(P<0.001),推拿治疗后P2X_3受体含量较模型组降低(P<0.05)。结论:推拿对腰椎间盘突出症大鼠有镇痛作用;推拿能够降低腰椎间盘突出症大鼠DRG神经元P2X_3受体的水平进而发挥镇痛效应。

Objective：To observe the effects of Tuina on lumbar disc herniation rat DRG neurons of P2X3 receptor to explore Tuina peripheral mechanisms of lumbar disc herniation analgesic effects. Methods ： Thirty - two SD rats were randomly divided into blank group, model group, sham group and Tuina group. The blank group received no treatment. The model and Tuina groups were the L - shaped stainless steel column to insert the intervertebral foramen causing DRG constant oppression. The sham - operated group except no L - shaped stainless steel column inserted intervertebral foramen. The rest step was the same as the model group＇ s. The Tuina group 4 d after modeling used massage therapy, and the rest did no therapy. After treatment, rats were detected mechanical response threshold （PWT）, paw withdrawal response latency （PWL）. Use the Western Blot method to observe changes in DRG neurons of P2X3 receptor content. Results ： For the blank group and sham - operated rats after modeling, PWT and PWL had no significant difference （ P 〉 0.05 ）. In the model group after modeling, PWT and PWL significantly decreased （P 〈 0. 001 ）. After Tuina therapy, compared with the model group, there was a significant difference （ P 〈 0.05 ）. Compared with the blank group and sham operation group, the content of P2X3 receptor neurons in model group was significantly higher （ P 〈 0. 001 ）. After Tuina therapy, P2X3 receptor content reducedcompared with the model group＇ s （ P 〈 0. 05 ）. Conclusion ： Tuina on lumbar disc herniation rats has analgesic effect. Tuina can reduce the lumbar disc herniation rats＇ DRG neurons of P2X3 receptor level and then play the analgesic effect.