鼻息肉VEGF-B基因启动子甲基化研究

The exploration of VEGF-B methylation in the nasal polyp

目的:探讨VEGF-B基因启动子在鼻息肉中甲基化状态。方法:应用MS-PCR检测28例实验组鼻息肉组织和12例对照组正常组织VEGF-B基因启动子甲基化状态,并对MS-PCR产物进行测序。结果:VEGFB启动子甲基化在28例鼻息肉中有9例阳性,19例阴性;在12例对照组织中8例阳性,4例阴性。鼻息肉组织和对照组织中VEGF-B基因启动子甲基化阳性率的差异有统计学意义(χ~2=4.096,P<0.05)。MS-PCR产物测序结果表明鼻息肉中存在VEGF-B基因启动子甲基化修饰。结论:鼻息肉中VEGF-B基因启动子可能存在低甲基化状态。推测鼻息肉VEGF-B基因甲基化调控着VEGF-B的表达,在鼻息肉的发生机制中占有重要地位。

Objective：The objective of this study is to investigate the methylation status of vascular endothelial cell growth factor B （VEGF-B） gene and to indentify the roles in pathogenesis, development and classification of nasal polyps. Method：The methylation status of VEGF-B gene of 28 nasal polyp tissues and 12 samples of inferior turbinate tissues were detected by methylationspecific-polymerase chain reaction （MS-PCR） and gene sequencing. Result：There was significant statistic diference between nasal polyp tissue group and control group （~.2= 4. 096, P^0.05）. The results of gene sequencing suggest that the VEGF-B gene promoter were hypomethylation status in the nasal polyps. Conclusion： Methylation status of VEGF-B promoter may play an important role in the pathoge- netic mechanism of nasal polyps.