内蒙古西部地区12份苜蓿根瘤菌株的16SrDNA鉴定

Identification of 12 Alfalfa Rhizobia Strains by 16SrDNA of Inner Mongolia western region

在内蒙古西部的4个地区(呼和浩特市,沙尔沁,海流图,达拉特旗),从9份豆科牧草材料(7份苜蓿属材料,2份草木樨属材料)上采集分离得到12份苜蓿根瘤菌株。通过16SrDNA鉴定,确定从草原3号杂花苜蓿(达拉特旗)和新疆大叶紫花苜蓿(海流图)上分离的2份菌株为固氮根瘤菌属(Rhizobium),而另外10份菌株均为苜蓿中华根瘤菌属(Sinorhizobium);采集于同一地点不同植物材料上的菌株大多数表现为亲缘关系较近。

12 Alfalfa Rhizobia Strains isolated from 9 legume forage material（7 Medicago material, 2 parts of the Melilotussuaveolens material） in the four western regions of Inner Mongolia （Hohhot, Shaerqin, Hailiutu, Dalad Banner）. Two strains isolated from the M. variaMartin, cv. Caoyuan No3. （Dalad Banner） and M. sativa L. cv. Xiniiangdaye （Hailiutu） were identified by 16SrDNA identification as Rhizobium, while the other 10 strains were Sinorhizobium;The strains showed close relationship which isolated from different materials but the same re-gions.