姜黄素对挤压综合征大鼠肾损伤及JNK信号通路活化抑制作用的实验研究

Experimental Study on Inhibition Effect of Curcumin on Renal Injury and Activation of JNK Signaling Pathway in Rats with Crush Syndrome

目的:观察姜黄素对挤压综合征大鼠肾损伤和JNK信号通路活化的抑制作用。方法:重物挤压建立大鼠挤压综合征模型,实验分为正常组、模型组、姜黄素低剂量组、姜黄素高剂量组,造模前2 h腹腔注射给药,观察各组大鼠肾组织及肌肉组织的病理变化,在造模6、12、24 h分别检测肾功指标(BUN、SCr),Western blot法检测各组大鼠肾组织JNK信号通路活化水平。结果:除正常组外,其余各组大鼠均可见肌肉和肾脏损伤,姜黄素组损伤较模型组为轻。与正常组比较,各造模组血清BUN、SCr水平明显升高(P<0.05),肾组织p-JNK表达明显增强(P<0.05),随挤压伤时间延长呈加重趋势;姜黄素组血清BUN、SCr水平较模型组降低(P<0.05),肾组织p-JNK表达明显减弱(P<0.05),高剂量组效果更显著。结论:姜黄素可以有效地减轻挤压综合征大鼠肾损伤,抑制肾组织JNK信号通路活化。

Objective ：To investigate the effect of Curcumin on renal injury and activation of JNK signaling pathway in rats of crush syndrome. Methods ： Models with crush syndrome were built by heavy extrusion, experi- ment was divided into normal, model and Curcumin low, high dose groups, Curcumin was given to rats by intrap- eritoneal injection before modeling 2 hours. The pathological changes of crushed muscle and kidney were ob- served, serum BUN and SCr levels were determined after modeling 6,12,24 hours, c - jun protein expression in kidney tissue was determined by Western blot method. Results：The muscle and kidney injuries were obvious in modeling rats except normal group, those in Curcumin groups were slighter than those in model group. To com- pare with normal group, the serum BNU and SCr levels were significantly raised in modeling rats, p - JNK ex- pression in renal tissue was enhanced significantly, these changes got worse with time ; serum BNU and SCr levels of Curcumin groups were lower than those in model group, p - JNK expression in renal tissue was abated, the effects of high dose group were more significant （ P 〈 0.05 ）. Conclusion： Curcumin can effectively reduce kidney injury in Crush syndrome rats,inhibit the activation of JNK signal pathway in renal tissue.