摘要
目的研究首发精神分裂症(first—episode schizophrenia,FES)患者外周血神经肽Y(neuropeptide Y,NPY)和催产素(oxytocin,OXT)的血浆浓度以及OXT mRNA的表达变化及其意义。方法采用酶联免疫吸附法(enzyme—linked immunosorbent assay,ELISA)检测55例FES患者和33例正常对照(healthy controls,HC)组血浆中NPY和OXT的浓度,用实时荧光定量PCR法(RT—qPCR)检测外周血淋巴细胞OXTmRNA的表达水平。结果FES患者血浆NPY的浓度[(1386.88±254.57)pg/ml]较HC组[(1140.62±266.63)pg/ml]显著升高(P〈0.01),且FES男性和女性患者[(1489.97±231.06)pg/ml和(1279.97±236.30)pg/ml]均高于同性别HC组[(1305.40±238.80)pg/ml和(965.55±165.45)pg/ml)](P〈0.05和P〈0.01)。FES患者血浆OXT浓度[(553.26±180.49)pg/ml]较HC组[(696.27±280.77)pg/ml]显著降低(P〈0.05),其中FES女性患者血浆OXT浓度[(597.49±178.63)pg/ml]显著低于HC组女性[(785.51±329.60)pg/ml](P〈0.05),两组问男性血浆OXT浓度比较差异无统计学意义(P〉0.05);FES患者外周血淋巴细胞OXTmRNA表达水平[0.2075(0.1653,0.3388)]较HC组[0.1615(0.1321,0.2200)]显著升高(P〈0.05),两组间女性OXTmRNA表达水平筹异无统计学意义(P〉0.05)。结论FES患者存在NPY和OXT的表达异常,血浆NPY和OXT的浓度变化对精神分裂症(schizophrenia,SZ)的诊断有一定指导意义。
Objective To investigate the clinical significance of plasma neuropeptide Y ( NPY ) and oxytocin (OXT) and the mRNA level of OXT gene in first-episode schizophrenia (FES) patients and healthy controls (HC). Methods The plasma concentration of NPY and OXT in 55 FES patients and 33 HC were measured by enzyme linked immunosorbent assay (ELISA) and the mRNA level of OXT gene were performed by real-time quantitative polymerase chain reaction (RT-qPCR) .Results The NPY levels in FES patients ( (1386.88±254.57)pg/ml) were significantly higher than that in HC ( ( 1 140.62±266.63) pg/ml) (P〈0.01) ,and both the males and females in FES patients (( 1 489.97±231.06)pg/ml and (1 279.97± 236.30) pg/ml) were higher than their counterparts in HC ( ( 1 305.40± 238.80) pg/ml and ( 965.55 ± 165.45 ) pg/ml ) (P〈 0.05, P〈 0.01 ). The OXT level in FES patients ( ( 553.26± 180.49 ) pg/ml ) was lower than that in HC ((696.27±280.77) pg/ml) with significant dift)rence (P〈0.05), especially the females FES patients ( (597.49±178.63)pg/ml vs (785.51±329.60)pg/ml) ; but the mRNA expression of OXT gene in FES patients (0.2075 (0. 1653,0.3388)) were significantly higher than that in HC (0. 1615 (0. 1321, 0.2200) ) (P〈0.05), and there were no differences between the females of the two groups(P〉0.05). Conclusion The FES patients presented abnormal expression of NPY and OXT,and the concentration changes of plasma NPY and OXT will provide referential significance for the diagnosis of schizophrenia(SZ).
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2017年第9期834-837,共4页
Chinese Journal of Behavioral Medicine and Brain Science
基金
国家自然科学基金项目(81771448)
湖南省自然科学基金项目(2015JJ4069)
关键词
首发精神分裂症
神经肽Y
催产素
酶联免疫吸附法
First-episode schizophrenia
Neuropeptide Y
Oxytocin
Enzyme-linked immunosorbent assay