维生素D受体基因多态性与藏族儿童结核病易感性研究

Study on vitamin D receptor gene polymorphisms and pulmonary tuberculosis susceptibility among Tibetan children in Qinghai-Tibetan plateau

目的探讨维生素D受体基因Fok I、BsmⅠ多态性与青藏高原藏族儿童结核病易感性的相关性。方法选取青海省妇女儿童医院2013-2015年的162例藏族儿童活动性肺结核患者作为病例组,167例有分枝杆菌接触史的同民族健康者作为对照组。采用聚合酶链反应-限制性片段长度多态性分析方法对两组研究对象进行维生素D受体基因FokⅠ、BsmⅠ多态性的检测,根据不同标本中分布结果进行统计学分析以研究两种多态性与青藏高原藏族儿童肺结核易感性的关系。结果病例组的FF、Ff与ff 3种基因型频率高于对照组,差异有统计学意义(P<0.05,OR=2.692,95%CI:1.349~5.373)。病例组的BB、Bb、bb3种基因型与对照组的相应基因型频率差异无统计学意义(P>0.05,OR=1.009,95%CI:0.560~1.816)。结论维生素D受体的FokⅠ多态性与高原藏族儿童肺结核易感性相关,其ff基因型可能是发病的风险因素,而与BsmⅠ多态性则无关。

Objective To study the correlation between vitamin D receptor（VDR）gene FokⅠ and BsmⅠ polymorphisms with pulmonary tuberculosis（PTB）susceptibility among Tibetan children in Qinghai-Tibetan plateau.Methods One hundred and sixtytwo Tibetan children cases of PTB in the Qinghai Provinicial Women and Children＇s Hospital during 2013 to 2015were selected as the cases group and 167 healthy Tibetan children with mycobacterial contact history as the healthy control group.The VDR gene FokⅠ and BsmⅠin the two groups were detected by PCR-RFLP.The association between two kinds of polymorphisms with PTB susceptibility were analyzed by adopting PCR-RFLP.Then the distribution results of different samples were performed the statistical analysis for conducting the relationship between the two kinds of polymorphisisms and PTB susceptibility of Tibetan children.Results The genotype frequencies of FF,Ff and ff in the cases group were higher than those in the control group,the difference was statistically significant（P〈0.05,OR：2.692,95%CI：1.349-5.373）.The genotype BB,Bb and bb had no statistically significant difference between the cases group and control group（P〉0.05,OR：1.009 95%CI：0.560-1.816）.Conclusion The VDR FokⅠ gene polymorphism is corrrelated with the PTB susceptibility among Tibetan children in Qinghai-Tibetan plateau,its genotype ff might be an onset risk factor.But the BsmⅠ polymorphism has no relationship with PTB onset.