强骨饮对假体周围骨溶解模型大鼠骨密度的影响及其作用机制研究

Effect of Qianggu Yin(强骨饮) on bone density of peri-prosthetic osteolysis rat models and its mechanism of action

目的:探讨强骨饮对假体周围骨溶解模型大鼠骨密度的影响及其作用机制。方法:选取30只成年雄性SD大鼠,随机分为空白组、模型组和强骨饮组,每组10只。空白组不进行造模;模型组和强骨饮组大鼠右侧股骨植入高密度聚乙烯颗粒和钛棒,制备聚乙烯颗粒诱导的假体周围骨溶解模型。造模手术3 d后,强骨饮组大鼠以制备好的强骨饮药液灌胃,每周3次,连续给药11周。空白组和模型组均不进行药物干预。造模后12周,取大鼠静脉血用ELISA法测定血清抗酒石酸酸性磷酸酶5b(tartrate resistant acid phosphatase 5b,TRACP5b)水平,取右侧股骨进行骨密度检测,并以ELISA法测定股骨组织中肿瘤坏死因子-α(tumor necrosis factorα,TNF-α)水平。结果:实验过程中各组大鼠日常行为无差别,精神状况良好,反应灵敏,体质量逐渐增加。模型组和强骨饮组大鼠术后1周切口愈合。实验全过程中各组均无大鼠死亡。3组大鼠血清TRACP5b水平比较,差异有统计学意义[(1.83±0.42)U·L^(-1),(2.40±0.43)U·L^(-1),(1.90±0.28)U·L^(-1),F=9.323,P=0.002]。模型组血清TRACP5b水平高于空白组和强骨饮组(P=0.001;P=0.002)。3组大鼠股骨骨密度比较,差异有统计学意义[(0.162±0.009)g·cm^(-2),(0.119±0.010)g·cm^(-2),(0.140±0.007)g·cm^(-2),F=74.323,P=0.000]。模型组股骨骨密度低于空白组和强骨饮组(P=0.000;P=0.000)。3组大鼠股骨组织中TNF-α水平比较,差异有统计学意义[(70.21±8.83)pg·mL^(-1),(175.32±14.95)pg·mL^(-1),(129.73±12.86)pg·mL^(-1),F=160.242,P=0.000]。模型组股骨组织TNF-α水平高于空白组和强骨饮组(P=0.000;P=0.000)。结论:强骨饮可以增加假体周围骨溶解模型大鼠的骨密度,其机制可能是通过减轻炎症反应,抑制破骨细胞活性,从而减少假体周围骨吸收。

Objective：To explore the effects of Qianggu Yin（ 强骨饮, QGY）on bone density of peri -prosthetic osteolysis rat models and its mechanism of action. Methods ： Thirty adult male SD rats were selected and randomly divided into blank group, model group and QGY group, 10 cases in each group. High density polyethylene（HDPE） particles and titanium rods were implanted into the right femurs of rats in model group and QGY group to build the peri - prosthetic osteolysis models induced by HDPE particles. At 3 days after the molding operation,the rats in QGY group were intragastric administrated with prepared QGY,3 times a week for consecutive 11 weeks. No drug intervention were performed on rats in blank group and model group. At 12 weeks after the modeling, the venous blood were fetched out and the serum levels of tartrate resistant acid phosphatase 5b（TRACPSb） were measured by using ELISA. The right femurs were taken from rats for detecting the bone densities, and the levels of tumor necrosis factor α （ TNF - α ） in femoral tissues were measured by using ELISA. Results：There were no difference in the daily actions of rats between the 3 groups during the course of experiment. The rats were in good mental states and rapid response and their body masses gradually increased. The incisions of rats in model group and QGY group were healed at 1 week after the surgery. No rats died in each group during the experiment. There was statistical difference in the serum levels of TRACP5b between the 3 groups （ 1.83 ＋/- 0.42,2.40 ＋/- 0.43,1.90 ＋/- 0.28 U/L, F = 9. 323, P = 0. 002 ）. The serum levels of TRACP5 b were higher in model group compared to blank group and QGY group （P = 0. 001 ;P = 0.002 ）. There was statistical difference in the femoral bone density between the 3 groups （0. 162 ＋/-0. 009,0.119 ＋/-0. 010,0. 140 ＋/-0. 007 g/cm（2） , F = 74. 323, P = 0. 000 ）. The femoral bone densities were lower in model group compared to blank group and QGY group（ P = 0. 000 ;P = 0. 000）. There was statistical difference in the TNF - α levels in femoral tissues between the 3 groups（70.21 ＋/- 8.83,175.32 ＋/- 14.95,129.73 ＋/- 12.86 pg,/ml, F = 160. 242 ,P =0. 000）. The TNF-α levels in femoral tissues were higher in model group compared to blank group and QGY group （P =0. 000;P = 0. 000 ）. Conclusion：QGY can alleviate the inflammatory reaction and inhibit the cytoactive of osteoclasts and accordingly reduce peri -prosthetic bone resorption ,which may be its mechanisms of action for increasing bone density of peri -prosthetic osteolysis rat models.