小立碗藓PpAGO7基因的克隆及表达分析

Cloning and Expression Analysis of PpAGO7 in Physcomitrella patens

该研究采用同源克隆的方法获得了小立碗藓AGO7蛋白编码基因PpAGO7,对PpAGO7基因序列特征进行生物信息学分析,并利用Real-time PCR方法分析PpAGO7基因的时空表达模式,为揭示小立碗藓中PpAGO7基因的生物学功能提供依据。结果表明:PpAGO7基因的全长cNDA为3 583bp,其中开放阅读框3 363bp,编码1 120个氨基酸,分子量123.38kD,等电点9.26,含有AGO蛋白典型的PAZ和PIWI结构域;PpAGO7基因在小立碗藓整个生活周期都有表达,且在茎叶体生长时期表达水平较高,但在原丝体时期表达水平较低。研究结果推测PpAGO7基因可能在茎叶体拟叶的生长发育过程中起作用。

By comparison to the amino acid sequence of OsAGO7 in rice with BLASTP tool,PpAGO7 gene was cloned fromPhyscomitrella patens; and the expression pattern was analyzed by real-time PCR.The full length sequence of PpAGO7 was 3 583 bp,containing a 3 363 bp open reading frame(ORF),which encoded a protein of 1 120 amino acids.Real-time PCR analysis showed that PpAGO7 gene was expressed throughout the whole life cycle of P.patens.The expression level of PpAGO7 gene in cormophyte period was higher than that in protonema period, and the expression level in the upper part of mimicry leaf was the highest.These results indicated that PpAGO7 gene may play an important role in the growth and development of mimicry leaf in cormophyte.