红发夫酵母有机硒最佳转化条件的研究

Optimum Transformation Conditions of Organic Selenium by Phaffia rhodozyma

[目的]优化红发夫酵母(Phaffia rhodozyma)有机硒转化条件。[方法]通过正交试验研究硒添加量、培养时间和装液量对红发夫酵母富硒的影响。[结果]红发夫酵母富硒的最适培养基为PDA培养基,亚硒酸钠的添加方式为分2次添加,在硒添加量20 mg/L、培养时间30 h、装液量80 m L/500 m L的条件下,红发夫酵母的生物量达10.62 g/L,有机硒转化率达63.2%。[结论]研究结果为红发夫酵母富硒的进一步研究提供了参考。

[Objective] The aim was to optimize the transformation conditions of organic selenium by Phaffia rhodozyma.[Method]The effect of selenium addition,culture time and liquid loading on selenium accumulation in Phaffia rhodozyma was studied by orthogonal experiment. [Result] The optimum medium for selenium enrichment was PDA medium,and the adding method was added in two times. Under the condition of selenium content at 20 mg/L and the liquid loading at 80 m L/500 m L incubating for 30 h,the biomass and transformation rate of organic selenium could reach 10. 62 g/L and 63. 2%,respectively.[Conclusion]The results can be regarded as the reference for further research on selenium enrichment in Phaffia rhodozyma.