盐生植物盐芥高效率嫁接体系的构建

Construction of an Efficient Grafting System for Halophyte Eutrema salsuginea

[目的]构建盐生植物盐芥高效率嫁接体系。[方法]利用拟南芥下胚轴水平切割的方法进行了盐芥的无菌嫁接。[结果]苗龄是影响嫁接成功与否的关键因素,萌发7~10 d的幼苗为最佳嫁接苗,苗龄过小或过大均不利于嫁接,同时不同的蔗糖浓度及铁盐也对嫁接有一定影响。[结论]盐芥嫁接体系的成功构建为进一步研究盐生植物地上与地下互作及长距离信号传递的耐逆分子机理提供了参考。

[Objective] The aim was to establish an efficient grafting system for halophyte Eutrema salsuginea. [Method] The sterile grafting of Eutrema salsuginea was realized bu using a new method of grafting which used the hypocotyl horizontal cutting method with a two edge razor blade. [Result]The size of the age of seedling was the key factor that affected the success of the graft; the germination 7-10 days of seedlings was the best grafting period,and too small or too large seedlings were not good for grafting. Furthermore,different concentrations of sucrose and molysite also had certain influence on grafting. [Conclusion] This method will be good for the study of between ground and underground interactions and the molecular mechanism of the long distance signal transmission of halophytes.