白花泡桐11个种源间亲缘关系的ISSR分析

Genetic Relationship of 11 Paulownia fortunei Individuals Detected by ISSR Analysis

[目的]对白花泡桐11个种源间亲缘关系进行ISSR分析。[方法]选取10个不同地理种源的白花泡桐个体及"绿树"(又名新桐)无性系组培苗为研究对象,采用ISSR分子标记进行分析。[结果]共筛选出22对有效引物,扩增出112条带,其中有67条多态带,多态性比例为59.82%。根据ISSR聚类分析结果,在遗传距离为0.280时,11份白花泡桐材料可分为6类,第1类为江西、福建种源个体;第2类为"绿树"无性系与湖南、湖北、河南、浙江种源个体;第3类为江苏种源个体;第4类为河北种源个体;第5类为广西种源个体;第6类为广东种源个体。[结论]筛选得到的引物可以有效地将不同种源加以区分,并且能准确地将"绿树"无性系从众多种源中鉴定出来,该研究结果一方面为白花泡桐的品种选育提供分子辅助育种的技术支持,另一方面也有助于"绿树"无性系的鉴定及知识产权的维护。

[Objective]The genetic relationship of 11 Paulownia fotunei individuals was detected by ISSR analysis.[Method]10 P. fortunei individuals of different provenances and Lüshu clones tissue culture seedlings were analyzed by ISSR. [Result]22 paris of effective primers were screened and 112 bands were amplified,including 67 polymorphic bands with a polymorphism ratio of 59. 82%. All these individuals could be divided into six groups when genetic distance was 0. 280 by ISSR cluster analysis. The first group was consisted of Jiangxi and Fujian provenances individuals. The second group was consisted of Lüshu clone,Hunan,Hubei,Henan and Zhejiang provenances individuals. The third group was consisted of Jiangsu provenances individual. The fourth group was consisted of Hebei provenances individual. The fifth group was consisted of Guangxi provenances individual,and the sixth group was consisted of Guangdong provenances individual. [Conclusion] These screened primers could effectively distinguish different provenances,and identify Lüshu clones accurately,which provide a molecular assisted breeding method for P. fortunei's breeding and it is helpful for authentication and protection of intellectual property of Lüshu clones.