11周龄硝酸甘油大鼠模型表面效度研究

Study on the surface validity of 11-week-old nitroglycerin rat model

目的本研究旨在11周龄硝酸甘油偏头痛模型大鼠头痛行为学的基础上结合临床偏头痛的头痛症状学对偏头痛大鼠模型的表面效度进行研究。方法根据体重及基础痛阈将36只大鼠随机分成四组,即正常组、模型组、西药组(苯甲酸利扎曲普坦)、中药组(川芎-天麻),每组9只。造模后,用高清摄像头记录大鼠行为学2 h。通过空白对照和药物反证治疗进行系统评价。结果造模后2 h内,模型组爬笼及理毛次数少于正常组,挠头次数多于正常组。西药组及中药组挠头次数少于模型组。结论挠头次数可以作为评价偏头痛大鼠模型表面效度的核心内容及判断药物疗效的指标。同时发现,11周龄偏头痛大鼠行为可能与人类偏头痛症状更相近。

Objective To study the surface validity of migraine rats on the basis of the headache behavioral science of 11-week-old nitroglycerin migraine rate model combined with the symptomatology of clinical migraine. Methods 36 rats were randomly divided into 4 groups according to body weight and basic pain threshold, namely normal group, model group, Western medicine group(Rizatriptan), and traditional Chinese medicine group(ligusticum wallichii-gastrodia elata), with 9 rats in each group. After modeling, the behavior of rats was recorded by high-definition camera for 2 hours. A systematic review was conducted by blank control and drug counterevidence therapy. Results Within 2 hours after modeling, the frequency of climbing cage and grooming in the model group was less than that in the normal group,and the frequency of scratching head was more than that in the normal group. The frequency of scratching head in the Western medicine group and Chinese medicine group was less than that in the model group. Conclusion Scratching head can be used as the core content of evaluating surface validity of migraine rats and the index of judging drug efficacy. At the same time, the behavior of 11-week-old migraine rats are found to be more likely to be associated with human migraine symptoms.