摘要
目的了解异鼠李素对LPS刺激下THP-1细胞炎症因子IL-6、MCP-1、TNF-α释放的影响及其抗炎特征。方法用PI单染色法检测其细胞存活率;用ELISA法检测THP-1细胞炎症因子IL-6、MCP-1、TNF-α释放。结果不同浓度的LPS均能使炎症因子IL-6释放增高,且24 h及48 h间水平无差异,但随着LPS刺激浓度的增高,细胞的坏死数量也会随之升高;不同浓度的异鼠李素能在不同时间点不同程度地抑制炎症因子MCP-1、TNF-α、IL-6的释放。结论异鼠李素能抑制LPS刺激下THP-1细胞炎症因子IL-6、TNF-α、MCP-1的释放,并呈浓度依赖性。
Objectives Our study is aim to investigate the influence of the isorhamnetin on the releasing of inflam-matory factor cytokines of the THP-1 cells. Methods PI single staining method was used to detect the cell survival rate. ELISA was used to detecte the concentrations of inflammatory cytokines (MCP-1、IL-6、TNF-α). Results The concentra-tion of IL-6 in THP-1 cells were increased after stimulated with LPS and there no difference between the 24 h group and 48 h group. The survival rate of THP-1 cells decreased as the concentration of LPS increased. The isorhamnetin in different concen-tration could inhibit the secretion of inflammatory cytokines in different time. Conclusion It is found in our study that isorh-amnetin may inhibit the secretion of MCP-1 , IL-6, TNF-α in THP-1 cells stimulated by LPS in a concentration dependent way.
出处
《广州医药》
2017年第5期9-13,共5页
Guangzhou Medical Journal
关键词
异鼠李素
脂多糖
炎症因子
抗炎反应
单核细胞
Isorhamnetion
Lipopolysaccharides
Inflammatory cytokines
Anti-inflammtion
Mononuclear cell