新型耐水解牙本质粘结剂的制备及其体外细胞毒性研究

The preparation and in vitro cytotoxicity study of new hydrolysis-resistant adhesive used for dentin bonding

目的:制备树脂—牙本质粘结的光固化聚氨酯丙烯酸酯粘结剂,并对其吸水值、溶解值及生物安全性能进行评价。方法:采用溶液聚合法合成聚醚、聚酯共混型聚氨酯,并辅以甲基丙烯酸甲酯、丙烯酸丁酯为稀释剂制备聚氨酯丙烯酸酯型粘结剂(PU),并对其进行吸水值、溶解值测定和细胞毒性评价,并与两种商品树脂粘结剂(Single Bond2、Adper Easy One)进行比较。结果:吸水值:PU<Adper Easy One<Single Bond2,溶解值:PU<Single Bond2<Adper Easy One;MTT结果显示对人牙髓细胞毒性:PU<Adper Easy One<Single Bond2。粘结剂浸泡时间的不同对其体外细胞毒性有一定影响,细胞毒性24 h<48 h<72 h。结论:与传统常用的丙烯酸酯类树脂粘结剂相比,PU型粘结剂具有较好的耐水解性能且生物相容性良好。

Objective： To prepare light cured polyurethane acrylate adhesive for dentin bonding and we focus on its water absorption/solubility and in vitro cytotoxicity. Methods： Polyether and polyester blend polyurethane has been prepared with solution polymerization process,along with methyl methacrylate（ MMA）,butyl acrylate（ BAM） as diluent preparation to obtain the polyurethane acrylate adhesive（ PU） used in the dentin bonding. Then we evaluated water absorption/solubility and cytotoxicity of PU adhesive,and compared with two commercial adhesives（ Single Bond2,Adper Easy One）. Results： Water absorption： PU 〈Adper Easy One 〈Single Bond 2,water solubility： PU〈 Single Bond2 〈Adper Easy One; In MTT assay,cytotoxicity of PU adhesive and two commercial adhesives exposed to human pulp cells was PU〈Adper Easy One〈Single Bond 2. Cytotoxicity was affected by the exposure time,24 h〈48 h〈72 h.Conclusions： PU adhesive shows good resistance to hydrolysis performance and good biocompatibility compared with the traditional acrylic ester resin adhesive.