黄芪多糖对上调ABCA1表达与泡沫细胞内胆固醇含量的关系分析

Analysis of Correlation Between Astragalus Polysaccharides with ABCA1 Up-regulation and Cholesterol Content in Foam Cells

目的:探讨黄芪多糖对ABCA1蛋白表达的影响。方法:将RAW264.7巨噬细胞诱导分化成泡沫细胞,用不同浓度黄芪多糖对其干预24h,酶法测定泡沫细胞内胆固醇酯含量,ELISA法检测细胞内ABCA1的蛋白表达。结果:对照组、10、20、50、100mg/L的APS作用于泡沫细胞24h后,细胞内胆固醇酯含量分别为45.15±3.57、37.67±2.26、34.59±3.51、28.57±10.34、15.92±3.86nmol/mg。各组ABCA1蛋白浓度为(6.02±6.12)、(15.86±9.15)、(54.76±14.20)、(75.39±10.09)、(97.56±13.25)ng/L。各APS组与对照组比较,差异具有统计学意义(P<0.01)。结论:黄芪多糖可能通过上调ABCA1的蛋白表达而降低泡沫细胞内胆固醇含量。

Objective：To investigate the effect of APS on the protein expression of ABCA1 in RAW264.7 macrophage-derived foam cells.Methods：RAW264.7 macrophages were incubated with APS（0,10,20,50,100mg/L）.The cholesterol content in foam cells was determined by enzyme digestion with different concentrations of astragalus polysaccharides for 24 h.The expression of ABCA1 was detected by ELISA.Results：In the control group,the contents of cholesterolester in foam cells were（45.15±3.57）,（37.67±2.26）,（34.59±3.51）,（28.57±10.34）,（15.92±3.86）nmol/mg respectively after the application of APS（0,10,20,50,100mg/L）for 24h（P0.01）.The concentration of protein in each group was（6.02±6.12）,（15.86±9.15）,（54.76±14.20）,（75.39±10.09）,（97.56±13.25）ng/L.Compared with the control group,the difference was significantly significant.Conclusion：APS may reduce the cholesterol content of foam cells by up-regulating ABCA1 protein expression.