肿瘤靶向的磁性荧光IR780-Fe_3O_4纳米颗粒用于循环肿瘤细胞的检测

Tumor Targeting of Fluorescent Magnetic IR780-Fe_3O_4 Nanoparticles for Detection of Circulating Tumor Cells

循环肿瘤细胞(Circulating tumor cells,CTCs)的简单、快速分离和检测是目前临床研究中面临的一项挑战。本研究制备了具有肿瘤靶向识别作用的磁性荧光IR780-Fe_3O_4纳米颗粒,并将其用于CTCs的分离和检测。通过电镜、荧光光谱仪和超导量子干涉仪对合成的IR780-Fe_3O_4纳米颗粒进行表征;采用激光共聚焦显微镜和流式细胞仪对IR780-Fe_3O_4纳米颗粒对肿瘤细胞和正常细胞的靶向效果进行了分析;利用激光共聚焦显微镜对IR780-Fe_3O_4纳米颗粒在MCF-7细胞中的位置进行定位;并根据IR780-Fe_3O_4纳米颗粒孵育后肿瘤细胞的荧光强度绘制标准曲线。研究结果表明,IR780-Fe_3O_4能很好地靶向多种CTCs。细胞定位实验进一步表明,IR780-Fe_3O_4主要靶向识别肿瘤细胞的线粒体。通过Fe_3O_4磁性纳米颗粒偶联IR780建立的这种方法可很好地区分肿瘤细胞和正常细胞,并对模拟血液中的CTCs进行了分离和检测。

The tumor targeted fluorescent magnetic IR780-Fe_3O_4 nanoparticles were prepared for separation and detection of circulating tumor cells（CTCs）.These IR780-Fe_3O_4 nanoparticles were characterized by electron microscopy,fluorescence spectrometer,and superconducting quantum interferometer.The targeting effect of IR780-Fe_3O_4 nanoparticles was analyzed on the tumor and normal cells by confocal microscope and flow cytometry,and the confocal microscope was used to target the location of IR780-Fe_3O_4 nanoparticles in MCF-7 cells.The standard curve was drawn and evaluated accorded to the IR780-Fe_3O_4 nanoparticles fluorescence intensity of tumor cells after incubation.The results showed that IR780-Fe_3O_4 nanoparticles could target a variety of CTCs.Furthermore,cellular localization experiment proved that IR780-Fe_3O_4 nanoparticles could target the mitochondria of tumor cells.With the method of coupling magnetic Fe_3O_4 nanoparticles,IR780 could well distinguish the tumor and normal cells,which could be used for separating and detecting the CTCs in simulated blood.