基于新型介质阻挡放电离子源的药物快速检测方法研究

Rapid Detection of Medicine Based on New Dielectric Barrier Discharge Ionization Mass Spectrometry

本研究将单电极放电技术的DBDI与质谱(MS)联用,快速检测了4种低极性的合成药物,结果表明,4种合成药物主要生成[M+H]^+分子离子。此外还利用DBDI-MS对草乌、制草乌切片进行快速分析,在草乌中检测到乌头碱、中乌头碱、脱氧乌头碱的[M+H]^+离子,以及[M+H-60]^+碎片离子;在制草乌中只检测到乌头碱、中乌头碱、脱氧乌头碱的[M+H-60]^+碎片离子。所测草乌中的标志性药效成分主要为双酯类生物碱,制草乌中的标志性药效成分主要为单酯类生物碱。新型DBDI为药物研究提供了一种新的、快速检测方法,具有十分重要的理论和实际应用意义,在药物研究领域具有极大的应用潜力。

Currently,rapid detection of effective components in synthetic drugs and herbal medicine remains an important and difficult issue of medicine research.A novel ionization technique,called dielectric barrier discharge ionization（DBDI）,has strong ionization ability,and is suitable for weak polar substances.Besides,this technique possesses many intrinsic advantages,such as simplicity,rapidity,no complicated sample pretreatment,etc.In this study,a new DBDI ion source,based on single electrode technique,was used to detect four weak polar synthetic drugs.The results showed that the protonated molecular ions [M＋H]~＋ of four weak polar synthetic drugs were observed obviously.What＇s more,the DBDI ion source was also used for the rapid analysis of Radix Aconiti and Radix Aconiti Preparata pieces without any sample pretreatment.The result showed that the protonated molecular ions [M＋H]~＋ and fragment ions [M＋H-60]~＋ of aconitine,hypaconitine,and mesaconitine were detected in Radix Aconiti.And only the fragment ions [M＋H-60]~＋ were detected in Radix Aconiti Preparat.The researches indicated that diester aconitine and monoester aconitine were the main effective components of Radix Aconiti Radix and Aconiti Preparat,respectively.The new DBDI ion source provided a fast and reliable method to identify effective components of medicine,showing a broad application prospects in synthetic drugs and herbal medicine research.