双低油菜籽不同添加水平对牦牛空肠和盲肠食糜消化酶活性的影响

Effects of different addition level of rapeseed in diet on the activity of digestive enzymes in jejunum and cecum chyme of yak

为揭示牦牛日粮中双低油菜籽不同添加量对其空肠和盲肠食糜消化酶活性的影响及各酶活性间的关系,试验采用年龄、性别和体况基本一致的生长期健康阉牦牛18头,随机分为3组,分别饲喂添加油菜籽为21.75%(H组)、10%(M组)和0%(L组)的精饲料日粮,每个处理组6个重复,每个重复单头饲喂。试验结果表明:(1)不同油菜籽添加量对空肠食糜淀粉酶(AMS)、脂肪酶(LPS)和羧甲基纤维素酶(CLS)活性无显著性影响(P>0.05),胰蛋白酶(TRS)活性H组显著高于M组和L组(P<0.05),H组较M组及L组分别高51.30%、45.08%;(2)不同油菜籽添加量盲肠食糜消化酶活性H组的AMS和CLS活性显著高于M组和L组(P<0.05),AMS活性分别较M组和L组高75.26%、72.22%,CLS活性分别较M组和L组高68.73%、52.48%;(3)由相关性可知,空肠食糜消化酶活性中,AMS与CLS活性极显著正相关(P<0.01);盲肠食糜消化酶活性中,AMS、LPS、TRS、CLS间均呈极显著正相关(P<0.01)。

The experiment was conducted to study the effect of different additions of canola rapeseed on the activity of digestive enzymes in jejunum and cecal chyme and the relationship among the activities of each enzyme.A total of 18 healthy castrated yaks with the same age,gender and body condition were randomly divided into 3 groups and fed with rapeseed for 21.75% （H） 10% （M）and 0 % （L） of the concentrate feed, six treatments were repeated for each treatment group, and each repeat was fed alone.The results showed thatThere was no significant effect of different rapeseed additions on the activi-ties of amylase （AMS） lipase （LPS）and cellulase （CLS） in jejunum （P 〉 0.05）.The trypsin （TRS） activity in H group was significantly higher 51.30%,45.08% than that in M group and L group.（2）The activities of AMS and CLS in H group were significantly higher than those in M and L groups （P 〈 0.05）.The AMS activity in H group was higher 75.26% and 72.22% than that of M group and L group, CLS activity was higher 68.73% and 52.48% than that in M group and L group.（3）There was a significant positive correlation between AMS and CLS activities in the digestive enzyme activity of jejunum chyme （P 〈 0.01） and there was a significant positive correlation between AMS, LPS,TRS and CLS in the digestive enzyme activity of cecal chyme （P 〈 0.01）.