西洋参中总皂苷的富集工艺研究

Enrichment of Total Saponins from Panacis Quinquefolii Radix

目的研究大孔吸附树脂富集西洋参总皂苷的工艺条件及参数,并测定富集后拟人参皂苷F_(11)含量。方法采用紫外分光光度法,以西洋参总皂苷含量为指标,考察上样量、洗脱溶剂及其用量、除杂溶剂及其用量等条件;同时利用超高效液相色谱-串联质谱法(UPLC-MS/MS)检测西洋参中总皂苷富集液中拟人参皂苷F_(11)含量。结果优选大孔吸附树脂富集工艺条件为:采用D101型大孔吸附树脂,上样浓度0.5 g/mL,上样体积0.5 BV,5BV水洗,3 BV 20%乙醇洗脱除杂,6 BV 70%乙醇洗脱富集,流速1 mL/min,得到富集液中西洋参总皂苷转移率为81.04%,纯度为73.75%;富集液中拟人参皂苷F_(11)含量为2.03±0.28 mg/g。结论优选工艺可较好地富集西洋参中人参总皂苷,富集液中拟人参皂苷F_(11)含量稳定,说明优化富集工艺稳定、可行。

Objective To study the enrichment and purification technology of total saponins from Panacis quinquefolii radix with macroporous resin and determine the content of pseudo-ginsenoside F11 in the enrichment eluate. Methods The effects of loading quantity of sample, eluation solvent and its volume and impurity removal solvent and its volume on the contents of total saponins in the eluate were investigated by ultraviolet-visible spectrophotometry. The contents of pseudo-ginsenoside F11 in the enrichment eluate were determined by ultra performance liquid chromatographytandem mass spectrometry （UPLC-MS/MS）. Results The optimum purification technology was as follows： D101 resin was adopted, the sample concentration was 0.5 mg/mL, the sample volume was 0.5 BV; the sample was eluted by 5 BV water, 3 BV 20 % ethanol and 6 BV 70 % ethanol successively, with flow rate of 1 mL/min. The transport rate of total saponins was up to 81.04 %, with purity of 73.75 %. The content of pseudo ginsenoside-F11 in the enrichment eluate was 2.03 ± 0.28 mg/g. Conclusion The process is effective for the enrichment of total saponins from Panacis quinquefolii radix and the content of Pseudo Ginsenoside-Fu in the enrichment eluate was stable.