禽流感灭活疫苗(H9亚型)子代通过母源抗体获得被动免疫力的效力试验

Efficacy Test of Passive Immunity Obtained by Maternal Antibody Against Avian Influenza Inactivated Vaccine(H9 Subtype)

为进一步了解疫苗在免疫持续期临界点时子代通过母源抗体获得被动免疫力的结果及疫苗使用时间,以实验室试制的3批禽流感灭活疫苗(H9亚型)进行母源抗体对子代鸡的被动免疫试验。取父母种鸡接种疫苗4个月后的产蛋进行孵化,分别对各批次雏鸡进行血清学和免疫攻毒试验。结果表明,随着日龄的增大,子代鸡的母源抗体逐渐消退。因此,在进行子代鸡的免疫时,应该根据种鸡免疫的时间进行灵活确定,在接种前,应该进行母源抗体效价测定,确定<4log2,才能进行免疫接种;如果没有条件检测母源抗体效价,建议在10日龄后接种,以避免母源抗体干扰免疫效果。

In order to further understand the critical point of vaccination at the critical stage of immunity, the off- spring obtained the passive immunity through the mother antibody and the time of vaccine use, the passive immuniza- tion test of the matemal antibody against the offsprings of chickens was carriedout with 3 batches of inactivated avian influenza vaccine （H9 subtype） produced in the laboratory in this papper. When parents vaccinated 4 months later~ the eggs were hatched and the serological and immunological tests were conducted on each batch of chicks. The results show that the maternal antibodies of progeny chickens gradually decreased as the age increased. Therefore, when car- rying out the immunity of the offspring chicken, it should be flexibly determined according to the time of the immuniza- tion of the breeder chickens. Before vaccination, the maternal antibody titer should be determined, and Only titer was less than 41og2 could be vaccinated; if there were no conditions to detect maternal antibody titer, vaccination should be done at greater than 10 days of age to avoid maternal antibodies interfering with the immune response.