听神经瘤临床特征与细胞免疫的相关性研究

Relationship between clinical characters of acoustic neuroma and cellular immunity

目的探讨T淋巴细胞在听神经瘤组织及外周血中的表达及其与临床特征之间的相关性。方法收集65例听神经瘤患者的临床资料,采集外周血,检测各亚型淋巴细胞数量。取肿瘤组织标本进行免疫组化染色,以8例正常颅神经为对照,并与临床资料进行相关分析。结果 65例患者外周血各亚型淋巴细胞数量与临床资料无显著相关性。肿瘤组织中,CD4^+和CD8^+T淋巴细胞数显著高于对照,且与术前听力水平呈负相关,CD8^+T淋巴细胞数与术后面神经功能呈负相关。40岁以下患者中,肿瘤组织CD4^+、CD8^+T淋巴细胞数显著高于高龄患者。Foxp3在对照组中未见明显表达,与患者术前听力水平呈负相关。结论 T淋巴细胞介导的细胞免疫在听神经瘤的发生发展及患者神经功能的保留和恢复中发挥了重要作用。

OBJECTIVE To explore the relationship between the clinical characteristics of acoustic neuroma and the number of T lymphocytes in tumor tissues. METHODS Clinical data of 65 patients diagnosed with VS were retrospectively collected. Peripheral blood samples of the patients were collected and the number of T lymphocytes were counted by flow cytometry. Immunohistochemistry was carried out with the fresh tumor samples compared with 8 normal cranial nerve controls. The expression level of each immune protein was analyzed its correlation with clinical characters was explored. RESULTS The CD3＋, CD4＋ and CD8＋ T lymphocytes in the peripheral blood are not significantly correlated with clinical data. In the tumor tissues, the number of CD4＋ and CD8＋ T lymphocytes are much larger than those in normal cranial nerves, and negatively correlated with preoperative hearing. The number of CD8＋ T lymphocytes has a negative relationship with postoperative facial nerve function. In addition, CD4 and CD8 expression levels in the tumor samples from patients younger than 40 are much higher than those in elder patients. Foxp3 is not detected in the normal cranial nerves. The number of Foxp3＋ T lymphocytes is negatively correlated with preoperative hearing. CONCLUSION The CD4＋ and CD8＋ T lymphocytes mediated cellular immunity play an important role in the development of acoustic neuroma, as well as the reservation and restoration of nerve function.