SMCC在不同溶液中的降解行为研究

A study on degradation behavior of SMCC in different solutions

目的:研究SMCC在不同溶液中的降解情况。方法:将SMCC分别用ACN、DMA和DMSO等有机溶剂溶解并制成一定浓度(约3 mg/ml)的储备液,再用不同的缓冲液稀释10倍并混匀作为供试品溶液,采用高效液相色谱法观察主峰的降解情况。结果:对于SMCC的水解,碱性环境以及DMSO或DMA的存在有显著的促进作用,而酸性环境以及ACN的存在则有一定的抑制作用。结论:交联反应可在弱碱环境下以DMA/DMSO作溶剂,而色谱分析则可在酸性环境下以ACN作溶剂。

Objective： To investigate the degradation phenomenon of SMCC in different solutions. Methods： Stock solutions of SMCC were prepared in ACN, DMA and DMSO, respectively, to give a final concentration of 3 mg/ml. Working solutions were prepared by 10-fold dilution of stock solutions in several buffer solutions. The degradation of SMCC was monitored by an HPLC method. Results： The hydrolysis of SMCC could be accelerated with the introduction of basic buffer solution or DMA/DMSO, while it could be hindered with the introduction of acidic solution or ACN. Conclusion： Cross-linking reaction could be performed in weakly alkaline condition by using solvent of DMA/DMSO, while HPLC analysis could be carried out in acidic condition by using solvent of ACN.