西藏亚东牦牛线粒体DNA分析及系统进化研究

Analysis on Phyletic Evolution and Mitochondrial DNA of Yadong Yak(Bos grunniens) in Tibet

应用基因测序技术对西藏亚东牦牛线粒体基因组进行测序,并对测序数据质控与修剪及基因组组装、精细注释、功能注释与分析。结果表明:亚东牦牛线粒体基因组大小约为15 389 bp,包含13个PCGs、21个tRNAs、2个rRNAs及1个D-loop,其A+T碱基含量为60.86%,G+C碱基含量为39.14%;编码基因(PCGs、tRNAs&rRNAs)片段总长度为14 495 bp,占基因组总长度的94.19%;同时,分析了亚东牦牛与其他11个物种间的进化关系,发现亚东牦牛与甘南牦牛归为一类,亲缘关系最近。西藏亚东牦牛线粒体DNA分析为研究牦牛群体遗传、起源与分子进化提供了技术参考。

The complete mitochondrial genome sequence of Yadong yak（Bos grunniens）in Tibet has been sequenced by gene sequencing technique,the sequencing data were analyzed by quality control and pruning,genome assembly,fine annotation, functional annotation and analysis. The results showed that the complete mitochondrial genome of Yadong yak in Tibet was 15389 bp, which contained 13 protein-coding genes,21 tRNA genes,2 rRNA genes and a non-coding control region（D-loop region）. The overall base pair composition were 60.86% A＋T and 39.14% G＋C,the total length of coding genes（PCGs,tRNAs ＆amp; rRNAs）were 14495 bp,accounting for 94.19% of the total length of genome. At the same time,the mtDNA of Yadong yak were analyzed with other evolutionary relationships between 11 species,the result showed that Yadong yak and Gannan yak were classified as a class. The mitogenome sequence of Yadong yak would contribute to better population genetics protection and evolution unders-tanding of Bos grunniens.