摘要
目的:明确绵头雪莲花提取物对UVA照射下的人成纤维细胞的保护作用。方法:制备绵头雪莲花浸膏,将培养的人成纤维细胞分为正常对照组、UVA照射组(10 J/cm^2)及UVA+药物组,CCK8法检测细胞增殖活性,并比较各组吸光度值。结果:UVA+药物组漂浮死细胞(低于5%)少于UVA照射组(约20%~30%);UVA+药物组(200μg/mL)吸光度值为1.16±0.22,高于UVA照射组的0.5±0.12,差异有统计学意义(P<0.05)。结论:绵头雪莲花提取物对UVA照射人成纤维细胞具有一定的保护作用。
Objective： To determine the protective effects of the extract from Saussurea laniceps on the proliferation of human fibroblasts induced by UVA. Methods： The extract from Saussurea laniceps was prepared and the cultured human fibroblasts were divided into normal control group,UVA group and UVA＋ Saussurea laniceps group. The cell proliferation activity was detected by CCK8 and the OD of cells was detected.Results： Dead cells in UVA＋ Saussurea laniceps group were less than 5%,which was less than that in UVA group（ 20% ~ 30%）. OD in UVA＋ Saussurea laniceps group was 1.16± 0.22,which was higher than that in UVA group（ 0.5±0.12）,with a significant difference（ P〈0.05）. Conclusion： The extract of Saussurea laniceps has protective effects on human fibroblasts proliferation induced by UVA.
作者
郑博文
阮光洪
尼玛此里
鲁茸达瓦
瞿红叶
黄相中
李冰
陈文雄
夏玉洁
邓丹琪
ZHENG Bowen RUAN Guanghong NI Macili LU Rongdawa QU Hongye HUANG Xiangzhong LI Bing CHENG Wenxiong XIA Yujie DENG Dangqi(Department of Dermatology, the Second People Hospital of Yunnan Province, the Fourth Affiliated Hospital of Kunming Medical University, Kunming 650021, China Department of tibetan Medicine, the People Hospital of Deqin County, Diqing 674500, China Department of Oral Rehabilitation, the Dental Hospital of Yunnan Province, Kunming 650101, China Key Laboratory of Chemistry, Ethnic Medicinal Resources, State Ethnic Affairs Commission ＆ Ministry of Education, School of Ethnic Medicine, Yunnan Minzu University, Kunming 650504, China Center for Drug Safety Evaluation, Kunming Institute of Zoology, Chinese Academy of Sci- ences, Kunming 650223, China Department of Dermatology, the Second Affiliated Hospital of Kunming Medical university, Kunming 650101, China)
出处
《中国麻风皮肤病杂志》
2017年第9期531-535,共5页
China Journal of Leprosy and Skin Diseases
基金
云南省科技厅-昆明医科大学应用基础研究联合专项资金项目(编号:2014FZ043)
