不同深度针刺“足三里”穴对慢性胃炎大鼠消化及免疫指标的影响

Effects of Acupuncture at Zusanli in Various Depths on Digestion and Immune Indices of Rats with Chronic Gastritis

目的:研究不同深度针刺"足三里"穴对慢性胃炎大鼠的治疗作用机制以及提高免疫系统功能的效果。方法:根据随机区组设计方法,将44只大鼠分为4组:正常对照组,造模组,浅刺组,深刺组。慢性胃炎大鼠模型采用碘乙酰胺溶液诱导制备。正常对照组不作任何处理,造模组用相同方法固定大鼠,但不做针刺处理,浅刺组、深刺组将毫针刺入不同深度后,分别测定各组大鼠的血清胃泌素、白介素含量及胃液p H值,观察胃部出血点及体重变化。结果:相对于正常对照组,造模组大鼠血清胃泌素浓度降低,针刺后深刺组大鼠血清胃泌素浓度较造模组有显著提高(P<0.01)且深刺组作用优于浅刺组(P<0.05);比较正常对照组、造模组、浅刺组、深刺组的白介素IL-1β,其差异均无统计学意义(P>0.05)。对正常对照组、造模组、浅刺组、深刺组的组间互相比较,p H值差异均无统计学意义(P>0.05)。结论:深刺大鼠"足三里"穴治疗慢性胃炎,相较于浅刺,有着更明显刺激机体内血清胃泌素释放的功能;针刺"足三里"穴对于调节慢性胃炎大鼠的血清白介素浓度及胃液p H值无明显影响。

Objective： To observe the effect of acupuncture at Zusanli in various depths on the mechanism of the treatment and on improving immune function of the rats with chronic gastritis. Methods： There were 44 rats randomized into a control group,a model group,a shallow-needling group and a deep-needling group. A chronic gastritis rats’ model was set up using iodine acetamide solution. The control group received no intervention. For the model group,the rats were fastened without any acupuncture. After insertion in the deep-needling and the shallow-needling group,the serum gastrin,interleukin content and p H value of gastric juice,the weight changed and the stomach bleeding spots in all 4 groups were observed. Results： In comparison with the control group,the serum gastrin concentration decreased in the model group,and the serum gastrin concentration in the deep-needling group significantly increased compared with that in the model group（ P 〈 0. 01）. The serum gastrin concentration of deep-needling group increased more than that of shallow-needling group; there was no statistically significant difference in both the concentration of interleukin IL-1β and the p H value in the control group,the model group,the deep-needling group and the shallow-needling group（ P 〉 0. 05）. Conclusion： Acupuncture in rats at the Zusanli acupoint deeply into the muscle layer,compared with shallowly at the subcutaneous level,has a more obvious stimulation on the body’s serum gastrin release function; meanwhile,there is no significant influence on regulation of rats with chronic gastritis and gastric juice concentration of serum interleukin p H by acupuncture in rats at the Zusanli acupoint.