补肾益髓方及其拆方对自身免疫性脑脊髓炎小鼠脑和脊髓中BDNF/TrkB的影响

Effects of Bushenyisui Formula and Its Decomposed Formulas on Expressions of BDNF/TrkB in Brain and Spinal Cord of Mice with Experimental Autoimmune Encephalomyelitis

目的:观察补肾益髓(Bu Shen Yi Sui,BSYS)方及其拆方补肾(Bu Shen,BS)和化痰活血(Hua Tan Huo Xue,HTHX)方对实验性自身免疫性脑脊髓炎(Experimental Autoimmune Encephalomyelitis,EAE)小鼠脑和脊髓中脑源性神经营养因子(Brain-derived Neurotrophic Factor,BDNF)与受体Trk B的影响。方法:将EAE造模小鼠于当天和第7天背部皮下注射髓鞘少突胶质细胞糖蛋白(Myelin Oligodendrocyte Glycoprotein,MOG)35-55抗原,并在免疫当天和第2天后腹腔注射百日咳毒素(Pertussis toxin,PTX)。每日对小鼠进行不同药物的灌胃,并于造模第20和40天分别取脑和脊髓,采用实时荧光定量RTPCR(qRT-PCR)与Western Blot法检测BDNF和Trk B的表达。结果:BSYS及其拆方BS方与HTHX方均可明显上调EAE小鼠脑和脊髓BDNF和Trk B mRNA与蛋白的表达,与模型组比较,差异均有统计学意义(P<0.05或P<0.01)。BSYS方作用优于其BS与HTHX方,但差异无统计学意义(P>0.05)。结论:BSYS及其拆方促进轴突修复的作用可能与增强BDNF/Trk B表达有关,BSYS全方更有显著趋势。

Objective： To observe effects of Bushenyisui（ BSYS） formula and its decomposed formulas,Bu Shen（ BS） and Hua Tan Huo Xue（ HTHX）,on the expressions of brain-derived neurotrophic factor（ BDNF） and TrkB in the brain and spinal cord of mice with experimental autoimmune encephalomyelitis（ EAE）. Methods： The EAE mice were injected subcutaneously with the myelin oligodendrocyte glycoprotein（ MOG）35-55 in the back on the 1st day and the 7th,and were injected intraperitoneally with pertussis toxin（ PTX）. All the mice were administered intragastrically with the different medicine or distilled water once a day. The brain and spinal cord of mice were removed for the examination on day 20 th and 40 th. The expressions of BDNF and Trk B mRNA and proteins were detected by real time fluorescent quantitative（ qRT-PCR） and Western Blot analysis. Results： The expressions of BDNF and Trk B in the brain and spinal cord of EAE mice were significantly up-regulated by BSYS and its decomposed BS and HTHX,compared with the EAE mice（ P 〈 0. 05）. The effective trend of BSYS on the above indexes was better than that of the decomposed BS and HTHX formulas,but there was no statistical significance（ P 〉 0. 05）. Conclusion： The effect of BSYS and its decomposed formulas,BS and HTHX,on promoting axonal repair might be related to enhance the expression of BDNF/Trk B,and that of BSYS was more significant.