病毒性心肌炎患者心肌细胞线粒体DNA缺失的定量分析

Quantitative analysis of mitochondrial DNA deletions in myocardial biopsy samples from patients with viral myocarditis

为探讨病毒性心肌炎 (VMC)患者心肌细胞线粒体 DNA(mt NDA)缺失突变情况及意义 ,用定量 PCR法检测 2 0例 VMC患者心肌细胞及其外周血淋巴细胞 mt DNA4 977碱基对 (mt DNA4 977)和 mt DNA74 36 碱基对 (mt D-NA74 36 )缺失率。取 10例健康意外死亡者心肌和 2 0例献血员外周血淋巴细胞作正常对照。结果显示 ,正常对照者和 VMC患者心肌细胞均存在 m t DNA4 977及 mt DNA74 36缺失 ,合计缺失率分别为 0 .176 %、0 .384 % ,二者差异显著 ,P<0 .0 5 ;VMC患者外周淋巴细胞 mt DNA缺失程度与心肌细胞呈一致性改变 ,且有良好的相关性 (r=0 .92 0 ,P<0 .0 0 1)。提示 mt DNA缺失可能是 VMC发病过程中重要的心肌损伤机制 ;外周淋巴细胞在研究心肌细胞 mt

To investigate the mitochondrial DNA(mtDNA) deletions in myocardial biopsy samples in patients with viral myocarditis(VMC),and the relation between mtDNA deletions and peripheral lymphocytes mtDNA 4977 base pair (mtDNA 4977 )and mtDNA 7436 base pair (mtDNA 7436 ) deletion rates of myocytes and lymphocytes were measured by the method of quantitative PCR in 20 VMC patients,30 control cases(10 myocardial samples from the healthy cases died of accident,20 blood samples of lymphoctes form the blood donators) Results showed that mtDNA 4977 and mtDNA 7436 deletions were observed in both controls (0 176%)and patients with VMC(0 384%),P<0 05 The degree of mtDNA deletions in peripheral lymphocyte was similar with and showed well correlation with that in myocardium(r=0 920,P<0 001) These suggest mtDNA deletions in myocardium might play an important role in the pathogenesis of VMC as well The value of peripheral lymphocyte in the study of myocardial mtDNA deletion needs to be further investigated