以菊粉为底物全细胞催化生产甘露醇

The whole cell catalytic production of mannitol using inulin as substrate

将Leuconostoc mesenteroides来源的甘露醇脱氢酶(mdh)及Mycobacterium vaccae来源的甲酸脱氢酶(fdh)的共表达载体pRSFDuet-mdh-fdh,与葡萄糖辅助蛋白(glf)的表达载体pZY507glf共转入大肠杆菌BL21(DE3)中,成功构建了一个可将果糖转化为甘露醇的全细胞催化剂E.coli BL21(DE3)/pRSFDuet-mdh-fdh pZY507glf。该全细胞催化剂在pH 6.5、温度30℃时,具有最高的转化效率,甘露醇的产率可达到0.91g/g。通过与高活力的菊粉酶协同作用,甘露醇的产率可达到0.93g/g。

A whole-cell biotransformation system E. coli BL21 （DE3）/pRSFDuet-mdh-fdh pZY507glf for mannitol production were established through the mannitol dehydrogenase （mdh） derived from Leuconostoc rnesenteroides and the formate dehydrogenase gene （fdh） derived from Mycobacteriurn vaccae pRSFDuet-mdh-fdh with the glucose facilitator protein （glf） pZY507glf co-expressed in E. coli BL21（DE3）. The yield of mannitol could reach to 0.91 g/g under the optimum conditions of pH 6.5 at 30 ℃. The yield of mannitol could reach to 0.93 g/g by whole catalyst with high activity inulinase.