蒙药材手掌参中总黄酮的提取工艺及抗氧化活性研究

Studies on the extracting and antioxidant activities of total flavonoids in Mongolian medicine Gymnadenia conopsea

目的优选手掌参总黄酮的最佳提取工艺,并建立其含量测定方法 ,同时对手掌参总黄酮的抗氧化活性进行研究。方法通过正交试验选择手掌参总黄酮的提取工艺;采用比色法以芦丁为对照测定手掌参中总黄酮的含量。采用羟基自由基清除法评价其抗氧化活性。结果最佳提取工艺为90%乙醇,料液比1∶20,回流提取2次,每次60 min;芦丁在0.1094~0.8752 mg范围内与吸光度呈良好的线性关系,平均回收率为98.8%,RSD为1.05%。手掌参总黄酮对羟基自由基有较强的清除作用。结论优选的提取工艺稳定可靠,含量测定方法简便,准确、重复性好,不同产地手掌参中总黄酮的含量差异较大。手掌参总黄酮具有一定的抗氧化活性。

Objective To optimize the extraction process of total flavonoids in （;ymnactenta conopsea, estaDnsn a method of its content determination and research its antioxidant activities. Methods The orthogonal experiments was used to optimize the extraction process of total flavonoids in Gymnadenia conopsea. The content of total flavonoids were determined using rutin as reference by colorimetric. The antioxidant activity was evaluated by hydroxyl free radical scavenging activity. Results The optimized extraction conditions were 90% ethanol as solvent, material -liquid ratio of 1：20, reflux extraction 2 times, each time 60 min. Rutin showed good linear relationship in the range of 0.1094-0.8752 mg, the average recovery was 98.8%, RSD was 1.05%. The total flavonoids possessed potential scavenging activity of hydroxyl free radical. Conclusion The optimum extraction process is stabie, reliable, the method of content determination is simple, accurate, reproducible. The content of total flavonoids is quite different in Gymnadenia conopsea of different areas. The total flavonoids in Gymnadenia conopsea have obvious antioxidant activities.